Article DsRNA virus Protozoan virus RNA directed DNA polymerase RNA directed RNA polymerase RNA isolation RNA polymerase RNA sequencing Totiviridae Transcriptome mining Trichomonas vaginalis Trichomonasvirus Virus discovery algorithm amino acid sequence amino terminal sequence bacteriophage bioinformatics controlled study deoxyribonuclease I high throughput analysis inflammation nonhuman open reading frame phylogenetic tree phylogeny polymerase chain reaction prevalence reagent screening test