Article Base Sequence Binding Sites Cloning, Molecular DNA determination DNA recombination DNA structure DNA, Bacterial DNA-protein interaction Escherichia coli Flexure angles Fluorescence Resonance Energy Transfer Gene Expression Genetic Vectors Genome, Bacterial Integration Host Factors Kinetics Models, Molecular Nucleic Acid Conformation Protein Binding Protein Conformation, alpha-Helical Protein Conformation, beta-Strand Protein Interaction Domains and Motifs Recombinant Proteins Single Molecule Imaging Substrate Specificity Thermodynamics X ray crystallography alpha helix atomic force microscopy