Amino Acid Sequence Animals Article Binding Sites Bond cleavage frequency Catalytic Domain Coleoptera Colorado potato beetle Humans Hydrolysis Insect α-amylase Protein Binding Sequence Homology, Amino Acid Subsite structure Substrate Specificity Swine Tenebrio affinity chromatography alpha-Amylases amino acid sequence amylase animal beetle binding affinity binding site catalysis controlled study enzyme active site enzyme analysis enzyme purification