5' Untranslated Regions 5' untranslated region 5′ UTRs Analysis workflow CAGE Customizable High-Throughput Nucleotide Sequencing High-throughput method High-throughput sequencing Open Reading Frames Open reading frame Open reading frames Protein Biosynthesis Protein coding regions Proteins RNA RNA-seq Rapid growth Ribo-seq Ribosomes TCP-seq Transcriptomes Translation Translation initiation User friendly genetics high throughput sequencing metabolism open reading frame protein synthesis