Quantitative Intracellular pH Determinations in Single Live Mammalian Spermatozoa Using the Ratiometric Dye SNARF-5F

Quantitative Intracellular pH Determinations in Single Live Mammalian Spermatozoa Using the Ratiometric Dye SNARF-5F

Intracellular pH (pHi) plays a crucial role in mammalian sperm physiology. However, it is a challenging task to acquire quantitative single sperm pHi images due to their small size and beating flagella. In this study, we established a robust pHi imaging system using the dual-emission ratiometric pH...

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Bibliographic Details
Main Authors: Julio C. Chávez, Alberto Darszon, Claudia L. Treviño, Takuya Nishigaki
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-01-01
Series:Frontiers in Cell and Developmental Biology
Subjects:
intracellular pH
alkalization
spermatozoa
dual emission
image splitter
ratiometric
Online Access:https://www.frontiersin.org/article/10.3389/fcell.2019.00366/full

Internet

https://www.frontiersin.org/article/10.3389/fcell.2019.00366/full

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