18S Ribosomal RNA Evaluation as Preanalytical Quality Control for Animal DNA

The 18S ribosomal RNA (rRNA) gene is present in all eukaryotic cells. In this study, we evaluated the use of this gene to verify the presence of PCR-amplifiable host (animal) DNA as an indicator of sufficient sample quality for quantitative real-time PCR (qPCR) analysis. We compared (i) samples from...

Full description

Bibliographic Details
Main Authors: Cory Ann Leonard, Marina L. Meli, Marilisa Novacco, Nicole Borel
Format: Article
Language:English
Published: Hindawi Limited 2016-01-01
Series:BioMed Research International
Online Access:http://dx.doi.org/10.1155/2016/6104323