A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography

A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography

Determining optimal conditions for the production of well diffracting crystals is a key step in every biocrystallography project. Here, a microfluidic device is described that enables the production of crystals by counter-diffusion and their direct on-chip analysis by serial crystallography at room...

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Bibliographic Details
Main Authors: Raphaël de Wijn, Oliver Hennig, Jennifer Roche, Sylvain Engilberge, Kevin Rollet, Pablo Fernandez-Millan, Karl Brillet, Heike Betat, Mario Mörl, Alain Roussel, Eric Girard, Christoph Mueller-Dieckmann, Gavin C. Fox, Vincent Olieric, José A. Gavira, Bernard Lorber, Claude Sauter
Format: Article
Language:English
Published: International Union of Crystallography 2019-05-01
Series:IUCrJ
Subjects:
macromolecule
crystallization
counter-diffusion
microfluidics
seeding
ligand soaking
trace fluorescent labeling
serial crystallography
room temperature
protein structure
ChipX3
Online Access:http://scripts.iucr.org/cgi-bin/paper?S2052252519003622

Internet

http://scripts.iucr.org/cgi-bin/paper?S2052252519003622

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