2passtools: two-pass alignment using machine-learning-filtered splice junctions increases the accuracy of intron detection in long-read RNA sequencing

2passtools: two-pass alignment using machine-learning-filtered splice junctions increases the accuracy of intron detection in long-read RNA sequencing

Abstract Transcription of eukaryotic genomes involves complex alternative processing of RNAs. Sequencing of full-length RNAs using long reads reveals the true complexity of processing. However, the relatively high error rates of long-read sequencing technologies can reduce the accuracy of intron ide...

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Bibliographic Details
Main Authors: Matthew T. Parker, Katarzyna Knop, Geoffrey J. Barton, Gordon G. Simpson
Format: Article
Language:English
Published: BMC 2021-03-01
Series:Genome Biology
Subjects:
Splicing
Long-read sequencing
Spliced alignment
RNA-seq
Gene expression
Transcriptome assembly
Online Access:https://doi.org/10.1186/s13059-021-02296-0

Internet

https://doi.org/10.1186/s13059-021-02296-0

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