In situ</font></i><font size="3"> genomic DNA extraction for PCR analysis of regions of interest in four plant species and one filamentous fungi

The extraction methods of genomic DNA are usually laborious and hazardous to human health and the environment by the use of organic solvents (chloroform and phenol). In this work a protocol for in situ extraction of genomic DNA by alkaline lysis is validated. It was used in order to amplify regions...

Full description

Bibliographic Details
Main Authors: Luis E. Rojas, Maritza Reyes, Naivy Pérez-Alonso, María I. Olóriz, Laisyn Posada-Pérez, Bárbara Ocaña, Orelvis Portal, Borys Chong-Pérez, Jorge L. Pérez Pérez
Format: Article
Language:English
Published: Universidad Central Marta Abreu de Las Villas 2014-07-01
Series:Biotecnología Vegetal
Online Access:https://revista.ibp.co.cu/index.php/BV/article/view/70