Combining 3D single molecule localization strategies for reproducible bioimaging
3D single molecule localization microscopy suffers from several experimental biases that degrade the resolution or localization precision. Here the authors present a dual-view detection scheme combining supercritical angle fluorescence and astigmatic imaging to obtain precise and unbiased 3D super r...
Main Authors: | , , , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2019-04-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-019-09901-8 |