Evaluating anthracycline cardiotoxicity associated single nucleotide polymorphisms in a paediatric cohort with early onset cardiomyopathy
Abstract Background Anthracyclines are a mainstay of chemotherapy. However, a relatively frequent adverse outcome of anthracycline treatment is cardiomyopathy. Multiple genetic studies have begun to dissect the complex genetics underlying cardiac sensitivity to the anthracycline drug class. A number...
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doaj-00a1c81a173c4ade91cf57973bb5ce842020-11-25T02:57:42ZengBMCCardio-Oncology2057-38042020-05-01611610.1186/s40959-020-00060-0Evaluating anthracycline cardiotoxicity associated single nucleotide polymorphisms in a paediatric cohort with early onset cardiomyopathyTimothy N. McOwan0Lauren A. Craig1Anne Tripdayonis2Kathy Karavendzas3Michael M. Cheung4Enzo R. Porrello5Rachel Conyers6David A. Elliott7Murdoch Children’s Research Institute, The Royal Children’s HospitalMurdoch Children’s Research Institute, The Royal Children’s HospitalMurdoch Children’s Research Institute, The Royal Children’s HospitalMurdoch Children’s Research Institute, The Royal Children’s HospitalMurdoch Children’s Research Institute, The Royal Children’s HospitalMurdoch Children’s Research Institute, The Royal Children’s HospitalMurdoch Children’s Research Institute, The Royal Children’s HospitalMurdoch Children’s Research Institute, The Royal Children’s HospitalAbstract Background Anthracyclines are a mainstay of chemotherapy. However, a relatively frequent adverse outcome of anthracycline treatment is cardiomyopathy. Multiple genetic studies have begun to dissect the complex genetics underlying cardiac sensitivity to the anthracycline drug class. A number of single nucleotide polymorphisms (SNPs) have been identified to be in linkage disequilibrium with anthracycline induced cardiotoxicity in paediatric populations. Methods Here we screened for the presence of SNPs resulting in a missense coding change in a cohort of children with early onset chemotherapy related cardiomyopathy. The SNP identity was evaluated by Sanger sequencing of PCR amplicons from genomic DNA of patients with anthracycline related cardiac dysfunction. Results All of the published SNPs were observed within our patient group. There was no correlation between the number of missense variants an individual carried with severity of disease. Furthermore, the time to cardiac disease onset post-treatment was not greater in those individuals carrying a high load of SNPs resulting from missense variants. Conclusions We conclude that previously identified missense SNPs are present within a paediatric cohort with early onset heart damage induced by anthracyclines. However, these SNPs require further replication cohorts and functional validation before being deployed to assess anthracycline cardiotoxicity risk in the clinic.http://link.springer.com/article/10.1186/s40959-020-00060-0Anthracycline induced cardiomyopathyGenetic variantsPaediatric cancers |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Timothy N. McOwan Lauren A. Craig Anne Tripdayonis Kathy Karavendzas Michael M. Cheung Enzo R. Porrello Rachel Conyers David A. Elliott |
spellingShingle |
Timothy N. McOwan Lauren A. Craig Anne Tripdayonis Kathy Karavendzas Michael M. Cheung Enzo R. Porrello Rachel Conyers David A. Elliott Evaluating anthracycline cardiotoxicity associated single nucleotide polymorphisms in a paediatric cohort with early onset cardiomyopathy Cardio-Oncology Anthracycline induced cardiomyopathy Genetic variants Paediatric cancers |
author_facet |
Timothy N. McOwan Lauren A. Craig Anne Tripdayonis Kathy Karavendzas Michael M. Cheung Enzo R. Porrello Rachel Conyers David A. Elliott |
author_sort |
Timothy N. McOwan |
title |
Evaluating anthracycline cardiotoxicity associated single nucleotide polymorphisms in a paediatric cohort with early onset cardiomyopathy |
title_short |
Evaluating anthracycline cardiotoxicity associated single nucleotide polymorphisms in a paediatric cohort with early onset cardiomyopathy |
title_full |
Evaluating anthracycline cardiotoxicity associated single nucleotide polymorphisms in a paediatric cohort with early onset cardiomyopathy |
title_fullStr |
Evaluating anthracycline cardiotoxicity associated single nucleotide polymorphisms in a paediatric cohort with early onset cardiomyopathy |
title_full_unstemmed |
Evaluating anthracycline cardiotoxicity associated single nucleotide polymorphisms in a paediatric cohort with early onset cardiomyopathy |
title_sort |
evaluating anthracycline cardiotoxicity associated single nucleotide polymorphisms in a paediatric cohort with early onset cardiomyopathy |
publisher |
BMC |
series |
Cardio-Oncology |
issn |
2057-3804 |
publishDate |
2020-05-01 |
description |
Abstract Background Anthracyclines are a mainstay of chemotherapy. However, a relatively frequent adverse outcome of anthracycline treatment is cardiomyopathy. Multiple genetic studies have begun to dissect the complex genetics underlying cardiac sensitivity to the anthracycline drug class. A number of single nucleotide polymorphisms (SNPs) have been identified to be in linkage disequilibrium with anthracycline induced cardiotoxicity in paediatric populations. Methods Here we screened for the presence of SNPs resulting in a missense coding change in a cohort of children with early onset chemotherapy related cardiomyopathy. The SNP identity was evaluated by Sanger sequencing of PCR amplicons from genomic DNA of patients with anthracycline related cardiac dysfunction. Results All of the published SNPs were observed within our patient group. There was no correlation between the number of missense variants an individual carried with severity of disease. Furthermore, the time to cardiac disease onset post-treatment was not greater in those individuals carrying a high load of SNPs resulting from missense variants. Conclusions We conclude that previously identified missense SNPs are present within a paediatric cohort with early onset heart damage induced by anthracyclines. However, these SNPs require further replication cohorts and functional validation before being deployed to assess anthracycline cardiotoxicity risk in the clinic. |
topic |
Anthracycline induced cardiomyopathy Genetic variants Paediatric cancers |
url |
http://link.springer.com/article/10.1186/s40959-020-00060-0 |
work_keys_str_mv |
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