In situ visualization of glucocerebrosidase in human skin tissue: zymography versus activity-based probe labeling[S]

In situ visualization of glucocerebrosidase in human skin tissue: zymography versus activity-based probe labeling[S]

Epidermal β-glucocerebrosidase (GBA1), an acid β-glucosidase normally located in lysosomes, converts (glucosyl)ceramides into ceramides, which is crucial to generate an optimal barrier function of the outermost skin layer, the stratum corneum (SC). Here we report on two developed in situ methods to...

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Bibliographic Details
Main Authors: Jeroen van Smeden, Irini M. Dijkhoff, Richard W.J. Helder, Hanin Al-Khakany, Daphne E.C. Boer, Anne Schreuder, Wouter W. Kallemeijn, Samira Absalah, Herman S. Overkleeft, Johannes M.F.G. Aerts, Joke A. Bouwstra
Format: Article
Language:English
Published: Elsevier 2017-12-01
Series:Journal of Lipid Research
Subjects:
ceramides
enzymology/enzyme regulation
fluorescence microscopy
Gaucher disease
human skin equivalents
in situ zymography
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520335562

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http://www.sciencedirect.com/science/article/pii/S0022227520335562

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