Room-temperature serial crystallography using a kinetically optimized microfluidic device for protein crystallization and on-chip X-ray diffraction

Room-temperature serial crystallography using a kinetically optimized microfluidic device for protein crystallization and on-chip X-ray diffraction

An emulsion-based serial crystallographic technology has been developed, in which nanolitre-sized droplets of protein solution are encapsulated in oil and stabilized by surfactant. Once the first crystal in a drop is nucleated, the small volume generates a negative feedback mechanism that lowers the...

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Bibliographic Details
Main Authors: Michael Heymann, Achini Opthalage, Jennifer L. Wierman, Sathish Akella, Doletha M. E. Szebenyi, Sol M. Gruner, Seth Fraden
Format: Article
Language:English
Published: International Union of Crystallography 2014-09-01
Series:IUCrJ
Subjects:
protein crystallization
X-ray diffraction
serial crystallography
microfluidic devices
Online Access:http://scripts.iucr.org/cgi-bin/paper?S2052252514016960

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http://scripts.iucr.org/cgi-bin/paper?S2052252514016960

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