Conventional Co-Housing Modulates Murine Gut Microbiota and Hematopoietic Gene Expression
Specific-pathogen-free (SPF) mice have improved hematopoietic characteristics relative to germ-free mice, however, it is not clear whether improvements in hematopoietic traits will continue when the level of microorganism exposure is further increased. We co-housed SPF C57BL/6 mice in a conventional...
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doaj-00fbe32dc0164d6388e4b9946d2f040d2020-11-25T03:40:08ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-08-01216143614310.3390/ijms21176143Conventional Co-Housing Modulates Murine Gut Microbiota and Hematopoietic Gene ExpressionJichun Chen0Shuling Zhang1Xingmin Feng2Zhijie Wu3Wendy Dubois4Vishal Thovarai5Sonia Ahluwalia6Shouguo Gao7Jinguo Chen8Tyler Peat9Shurjo K. Sen10Giorgio Trinchieri11Neal S. Young12Beverly A. Mock13Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USALaboratory of Cancer Biology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USAHematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USAHematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USALaboratory of Cancer Biology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USABasic Science Program, Frederick National Laboratory for Cancer Research, Frederick, MD 21702, USABasic Science Program, Frederick National Laboratory for Cancer Research, Frederick, MD 21702, USAHematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USACenter for Human Immunology and Autoimmunity, National Institutes of Health, Bethesda, MD 20892, USALaboratory of Cancer Biology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USABasic Science Program, Frederick National Laboratory for Cancer Research, Frederick, MD 21702, USACancer and Inflammation Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USAHematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USALaboratory of Cancer Biology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USASpecific-pathogen-free (SPF) mice have improved hematopoietic characteristics relative to germ-free mice, however, it is not clear whether improvements in hematopoietic traits will continue when the level of microorganism exposure is further increased. We co-housed SPF C57BL/6 mice in a conventional facility (CVT) and found a significant increase in gut microbiota diversity along with increased levels of myeloid cells and T cells, especially effector memory T cells. Through single cell RNA sequencing of sorted KL (c-Kit<sup>+</sup>Lin<sup>−</sup>) cells, we imputed a decline in long-term hematopoietic stem cells and an increase in granulocyte-monocyte progenitors in CVT mice with up-regulation of genes associated with cell survival. Bone marrow transplantation through competitive repopulation revealed a significant increase in KSL (c-Kit<sup>+</sup>Sca-1<sup>+</sup>Lin<sup>−</sup>) cell reconstitution in recipients of CVT donor cells which occurred when donors were co-housed for both one and twelve months. However, there was minimal to no gain in mature blood cell engraftment in recipients of CVT donor cells relative to those receiving SPF donor cells. We conclude that co-housing SPF mice with mice born in a conventional facility increased gut microbiota diversity, augmented myeloid cell production and T cell activation, stimulated KSL cell reconstitution, and altered hematopoietic gene expression.https://www.mdpi.com/1422-0067/21/17/6143micespecific pathogen freeconventional co-housinggut microbiotasingle cell RNA-seqhematopoietic stem and progenitor cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jichun Chen Shuling Zhang Xingmin Feng Zhijie Wu Wendy Dubois Vishal Thovarai Sonia Ahluwalia Shouguo Gao Jinguo Chen Tyler Peat Shurjo K. Sen Giorgio Trinchieri Neal S. Young Beverly A. Mock |
spellingShingle |
Jichun Chen Shuling Zhang Xingmin Feng Zhijie Wu Wendy Dubois Vishal Thovarai Sonia Ahluwalia Shouguo Gao Jinguo Chen Tyler Peat Shurjo K. Sen Giorgio Trinchieri Neal S. Young Beverly A. Mock Conventional Co-Housing Modulates Murine Gut Microbiota and Hematopoietic Gene Expression International Journal of Molecular Sciences mice specific pathogen free conventional co-housing gut microbiota single cell RNA-seq hematopoietic stem and progenitor cells |
author_facet |
Jichun Chen Shuling Zhang Xingmin Feng Zhijie Wu Wendy Dubois Vishal Thovarai Sonia Ahluwalia Shouguo Gao Jinguo Chen Tyler Peat Shurjo K. Sen Giorgio Trinchieri Neal S. Young Beverly A. Mock |
author_sort |
Jichun Chen |
title |
Conventional Co-Housing Modulates Murine Gut Microbiota and Hematopoietic Gene Expression |
title_short |
Conventional Co-Housing Modulates Murine Gut Microbiota and Hematopoietic Gene Expression |
title_full |
Conventional Co-Housing Modulates Murine Gut Microbiota and Hematopoietic Gene Expression |
title_fullStr |
Conventional Co-Housing Modulates Murine Gut Microbiota and Hematopoietic Gene Expression |
title_full_unstemmed |
Conventional Co-Housing Modulates Murine Gut Microbiota and Hematopoietic Gene Expression |
title_sort |
conventional co-housing modulates murine gut microbiota and hematopoietic gene expression |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2020-08-01 |
description |
Specific-pathogen-free (SPF) mice have improved hematopoietic characteristics relative to germ-free mice, however, it is not clear whether improvements in hematopoietic traits will continue when the level of microorganism exposure is further increased. We co-housed SPF C57BL/6 mice in a conventional facility (CVT) and found a significant increase in gut microbiota diversity along with increased levels of myeloid cells and T cells, especially effector memory T cells. Through single cell RNA sequencing of sorted KL (c-Kit<sup>+</sup>Lin<sup>−</sup>) cells, we imputed a decline in long-term hematopoietic stem cells and an increase in granulocyte-monocyte progenitors in CVT mice with up-regulation of genes associated with cell survival. Bone marrow transplantation through competitive repopulation revealed a significant increase in KSL (c-Kit<sup>+</sup>Sca-1<sup>+</sup>Lin<sup>−</sup>) cell reconstitution in recipients of CVT donor cells which occurred when donors were co-housed for both one and twelve months. However, there was minimal to no gain in mature blood cell engraftment in recipients of CVT donor cells relative to those receiving SPF donor cells. We conclude that co-housing SPF mice with mice born in a conventional facility increased gut microbiota diversity, augmented myeloid cell production and T cell activation, stimulated KSL cell reconstitution, and altered hematopoietic gene expression. |
topic |
mice specific pathogen free conventional co-housing gut microbiota single cell RNA-seq hematopoietic stem and progenitor cells |
url |
https://www.mdpi.com/1422-0067/21/17/6143 |
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