Detection of viable Salmonella in ice cream by TaqMan real-time polymerase chain reaction assay combining propidium monoazide

Detection of viable Salmonella in ice cream by TaqMan real-time polymerase chain reaction assay combining propidium monoazide

Real-time polymerase chain reaction (PCR) allows rapid detection of Salmonella in frozen dairy products, but it might cause a false positive detection result because it might amplify DNA from dead target cells as well. In this study, Salmonella-free frozen ice cream was initially inoculated with hea...

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Bibliographic Details
Main Authors: Yuexia Wang, Ming Yang, Shuchun Liu, Wanyi Chen, Biao Suo
Format: Article
Language:English
Published: Elsevier 2015-09-01
Series:Journal of Food and Drug Analysis
Subjects:
ice cream
propidium monoazide
Salmonella
TaqMan real-time PCR
viable cells
Online Access:http://www.sciencedirect.com/science/article/pii/S1021949815000460

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http://www.sciencedirect.com/science/article/pii/S1021949815000460

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