Application of high-throughput 5′P sequencing for the study of co-translational mRNA decay
Summary: mRNA degradation is connected to the translation process up to the degree that 5′-3′ mRNA degradation follows the last translating ribosome. To study 5′-3′co-translational mRNA decay and the associated ribosome dynamics, here we present an improved high-throughput 5′P degradome RNA sequenci...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721001544 |