UNC93B1 mediates host resistance to infection with Toxoplasma gondii.
UNC93B1 associates with Toll-Like Receptor (TLR) 3, TLR7 and TLR9, mediating their translocation from the endoplasmic reticulum to the endolysosome, hence allowing proper activation by nucleic acid ligands. We found that the triple deficient '3d' mice, which lack functional UNC93B1, are hy...
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2010-08-01
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Series: | PLoS Pathogens |
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doaj-019e1088e0dc48ad9a82bf36bcfa865c2020-11-25T00:12:14ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742010-08-0168e100107110.1371/journal.ppat.1001071UNC93B1 mediates host resistance to infection with Toxoplasma gondii.Mariane B MeloPia KasperkovitzAnna CernyStephanie Könen-WaismanEvelyn A Kurt-JonesEgil LienBruce BeutlerJonathan C HowardDouglas T GolenbockRicardo T GazzinelliUNC93B1 associates with Toll-Like Receptor (TLR) 3, TLR7 and TLR9, mediating their translocation from the endoplasmic reticulum to the endolysosome, hence allowing proper activation by nucleic acid ligands. We found that the triple deficient '3d' mice, which lack functional UNC93B1, are hyper-susceptible to infection with Toxoplasma gondii. We established that while mounting a normal systemic pro-inflammatory response, i.e. producing abundant MCP-1, IL-6, TNFα and IFNγ, the 3d mice were unable to control parasite replication. Nevertheless, infection of reciprocal bone marrow chimeras between wild-type and 3d mice with T. gondii demonstrated a primary role of hemopoietic cell lineages in the enhanced susceptibility of UNC93B1 mutant mice. The protective role mediated by UNC93B1 to T. gondii infection was associated with impaired IL-12 responses and delayed IFNγ by spleen cells. Notably, in macrophages infected with T. gondii, UNC93B1 accumulates on the parasitophorous vacuole. Furthermore, upon in vitro infection the rate of tachyzoite replication was enhanced in non-activated macrophages carrying mutant UNC93B1 as compared to wild type gene. Strikingly, the role of UNC93B1 on intracellular parasite growth appears to be independent of TLR function. Altogether, our results reveal a critical role for UNC93B1 on induction of IL-12/IFNγ production as well as autonomous control of Toxoplasma replication by macrophages.http://europepmc.org/articles/PMC2928809?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mariane B Melo Pia Kasperkovitz Anna Cerny Stephanie Könen-Waisman Evelyn A Kurt-Jones Egil Lien Bruce Beutler Jonathan C Howard Douglas T Golenbock Ricardo T Gazzinelli |
spellingShingle |
Mariane B Melo Pia Kasperkovitz Anna Cerny Stephanie Könen-Waisman Evelyn A Kurt-Jones Egil Lien Bruce Beutler Jonathan C Howard Douglas T Golenbock Ricardo T Gazzinelli UNC93B1 mediates host resistance to infection with Toxoplasma gondii. PLoS Pathogens |
author_facet |
Mariane B Melo Pia Kasperkovitz Anna Cerny Stephanie Könen-Waisman Evelyn A Kurt-Jones Egil Lien Bruce Beutler Jonathan C Howard Douglas T Golenbock Ricardo T Gazzinelli |
author_sort |
Mariane B Melo |
title |
UNC93B1 mediates host resistance to infection with Toxoplasma gondii. |
title_short |
UNC93B1 mediates host resistance to infection with Toxoplasma gondii. |
title_full |
UNC93B1 mediates host resistance to infection with Toxoplasma gondii. |
title_fullStr |
UNC93B1 mediates host resistance to infection with Toxoplasma gondii. |
title_full_unstemmed |
UNC93B1 mediates host resistance to infection with Toxoplasma gondii. |
title_sort |
unc93b1 mediates host resistance to infection with toxoplasma gondii. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Pathogens |
issn |
1553-7366 1553-7374 |
publishDate |
2010-08-01 |
description |
UNC93B1 associates with Toll-Like Receptor (TLR) 3, TLR7 and TLR9, mediating their translocation from the endoplasmic reticulum to the endolysosome, hence allowing proper activation by nucleic acid ligands. We found that the triple deficient '3d' mice, which lack functional UNC93B1, are hyper-susceptible to infection with Toxoplasma gondii. We established that while mounting a normal systemic pro-inflammatory response, i.e. producing abundant MCP-1, IL-6, TNFα and IFNγ, the 3d mice were unable to control parasite replication. Nevertheless, infection of reciprocal bone marrow chimeras between wild-type and 3d mice with T. gondii demonstrated a primary role of hemopoietic cell lineages in the enhanced susceptibility of UNC93B1 mutant mice. The protective role mediated by UNC93B1 to T. gondii infection was associated with impaired IL-12 responses and delayed IFNγ by spleen cells. Notably, in macrophages infected with T. gondii, UNC93B1 accumulates on the parasitophorous vacuole. Furthermore, upon in vitro infection the rate of tachyzoite replication was enhanced in non-activated macrophages carrying mutant UNC93B1 as compared to wild type gene. Strikingly, the role of UNC93B1 on intracellular parasite growth appears to be independent of TLR function. Altogether, our results reveal a critical role for UNC93B1 on induction of IL-12/IFNγ production as well as autonomous control of Toxoplasma replication by macrophages. |
url |
http://europepmc.org/articles/PMC2928809?pdf=render |
work_keys_str_mv |
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