The human origin recognition complex is essential for pre-RC assembly, mitosis, and maintenance of nuclear structure

The human origin recognition complex is essential for pre-RC assembly, mitosis, and maintenance of nuclear structure

The origin recognition complex (ORC) cooperates with CDC6, MCM2-7, and CDT1 to form pre-RC complexes at origins of DNA replication. Here, using tiling-sgRNA CRISPR screens, we report that each subunit of ORC and CDC6 is essential in human cells. Using an auxin-inducible degradation system, we create...

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Bibliographic Details
Main Authors: Hsiang-Chen Chou, Kuhulika Bhalla, Osama EL Demerdesh, Olaf Klingbeil, Kaarina Hanington, Sergey Aganezov, Peter Andrews, Habeeb Alsudani, Kenneth Chang, Christopher R Vakoc, Michael C Schatz, W Richard McCombie, Bruce Stillman
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2021-02-01
Series:eLife
Subjects:
initiation of DNA replication
origin recognition complex
CDC6
pre-replicative complex
mitosis
CRISPR-Cas9 gene editing
Online Access:https://elifesciences.org/articles/61797

Internet

https://elifesciences.org/articles/61797

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