Persimmon tannin promoted macrophage reverse cholesterol transport through inhibiting ERK1/2 and activating PPARγ both in vitro and in vivo

Persimmon tannin promoted macrophage reverse cholesterol transport through inhibiting ERK1/2 and activating PPARγ both in vitro and in vivo

The purpose of this study was to investigate whether persimmon tannin is associated with cholesterol efflux and macrophage-reverse cholesterol transport (RCT). In J774A.1 macrophage cells, persimmon tannin could inhibit cellular cholesterol accumulation and promote 22-NBD-cholesterol efflux through...

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Main Authors: Zhenzhen Ge, Mengying Zhang, Xiangyi Deng, Wei Zhu, Kaikai Li, Chunmei Li
Format: Article
Language:English
Published: Elsevier 2017-11-01
Series:Journal of Functional Foods
Subjects:
Persimmon tannin
Macrophage RCT
Phosphorylation of ERK1/2
PPARγ
Cholesterol efflux
Online Access:http://www.sciencedirect.com/science/article/pii/S1756464617305352
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spelling doaj-020218d634254b7ebc757a36d94f4ac62021-04-30T07:11:44ZengElsevierJournal of Functional Foods1756-46462017-11-0138338348Persimmon tannin promoted macrophage reverse cholesterol transport through inhibiting ERK1/2 and activating PPARγ both in vitro and in vivoZhenzhen Ge0Mengying Zhang1Xiangyi Deng2Wei Zhu3Kaikai Li4Chunmei Li5College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Food Science (Huazhong Agricultural University), Ministry of Education, China; Corresponding author at: College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.The purpose of this study was to investigate whether persimmon tannin is associated with cholesterol efflux and macrophage-reverse cholesterol transport (RCT). In J774A.1 macrophage cells, persimmon tannin could inhibit cellular cholesterol accumulation and promote 22-NBD-cholesterol efflux through inhibiting the phosphorylation of ERK1/2 and up-regulating the protein levels of PPARγ. Macrophage RCT in vivo was evaluated by injecting 22-NBD-cholesterol-loaded J774A.1 macrophages intraperitoneally into C57BL/6J mice. Administration of persimmon tannin significantly (P < 0.05) decreased the cholesterol concentration in both serum and liver, and increased faecal cholesterol excretion compared with the high-cholesterol group. In transcriptional levels, persimmon tannin enhanced the expression of cholesterol transport-related genes (ABCA1, LCAT, ABCG5/G8, NPC1L1 and CYP7A1) and their upstream nuclear receptors (PPARγ, PPARα and LXRα). Moreover, the regulation of persimmon tannin on RCT-related genes might be mediated by its inhibition on ERK1/2 in mice. Therefore, persimmon tannin promoted macrophage reverse cholesterol transport through the regulation on ERK1/2-PPARγ signaling pathway both in vitro and in vivo.http://www.sciencedirect.com/science/article/pii/S1756464617305352Persimmon tanninMacrophage RCTPhosphorylation of ERK1/2PPARγCholesterol efflux
collection DOAJ
language English
format Article
sources DOAJ
author Zhenzhen Ge
Mengying Zhang
Xiangyi Deng
Wei Zhu
Kaikai Li
Chunmei Li
spellingShingle Zhenzhen Ge
Mengying Zhang
Xiangyi Deng
Wei Zhu
Kaikai Li
Chunmei Li
Persimmon tannin promoted macrophage reverse cholesterol transport through inhibiting ERK1/2 and activating PPARγ both in vitro and in vivo
Journal of Functional Foods
Persimmon tannin
Macrophage RCT
Phosphorylation of ERK1/2
PPARγ
Cholesterol efflux
author_facet Zhenzhen Ge
Mengying Zhang
Xiangyi Deng
Wei Zhu
Kaikai Li
Chunmei Li
author_sort Zhenzhen Ge
title Persimmon tannin promoted macrophage reverse cholesterol transport through inhibiting ERK1/2 and activating PPARγ both in vitro and in vivo
title_short Persimmon tannin promoted macrophage reverse cholesterol transport through inhibiting ERK1/2 and activating PPARγ both in vitro and in vivo
title_full Persimmon tannin promoted macrophage reverse cholesterol transport through inhibiting ERK1/2 and activating PPARγ both in vitro and in vivo
title_fullStr Persimmon tannin promoted macrophage reverse cholesterol transport through inhibiting ERK1/2 and activating PPARγ both in vitro and in vivo
title_full_unstemmed Persimmon tannin promoted macrophage reverse cholesterol transport through inhibiting ERK1/2 and activating PPARγ both in vitro and in vivo
title_sort persimmon tannin promoted macrophage reverse cholesterol transport through inhibiting erk1/2 and activating pparγ both in vitro and in vivo
publisher Elsevier
series Journal of Functional Foods
issn 1756-4646
publishDate 2017-11-01
description The purpose of this study was to investigate whether persimmon tannin is associated with cholesterol efflux and macrophage-reverse cholesterol transport (RCT). In J774A.1 macrophage cells, persimmon tannin could inhibit cellular cholesterol accumulation and promote 22-NBD-cholesterol efflux through inhibiting the phosphorylation of ERK1/2 and up-regulating the protein levels of PPARγ. Macrophage RCT in vivo was evaluated by injecting 22-NBD-cholesterol-loaded J774A.1 macrophages intraperitoneally into C57BL/6J mice. Administration of persimmon tannin significantly (P < 0.05) decreased the cholesterol concentration in both serum and liver, and increased faecal cholesterol excretion compared with the high-cholesterol group. In transcriptional levels, persimmon tannin enhanced the expression of cholesterol transport-related genes (ABCA1, LCAT, ABCG5/G8, NPC1L1 and CYP7A1) and their upstream nuclear receptors (PPARγ, PPARα and LXRα). Moreover, the regulation of persimmon tannin on RCT-related genes might be mediated by its inhibition on ERK1/2 in mice. Therefore, persimmon tannin promoted macrophage reverse cholesterol transport through the regulation on ERK1/2-PPARγ signaling pathway both in vitro and in vivo.
topic Persimmon tannin
Macrophage RCT
Phosphorylation of ERK1/2
PPARγ
Cholesterol efflux
url http://www.sciencedirect.com/science/article/pii/S1756464617305352
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AT mengyingzhang persimmontanninpromotedmacrophagereversecholesteroltransportthroughinhibitingerk12andactivatingppargbothinvitroandinvivo
AT xiangyideng persimmontanninpromotedmacrophagereversecholesteroltransportthroughinhibitingerk12andactivatingppargbothinvitroandinvivo
AT weizhu persimmontanninpromotedmacrophagereversecholesteroltransportthroughinhibitingerk12andactivatingppargbothinvitroandinvivo
AT kaikaili persimmontanninpromotedmacrophagereversecholesteroltransportthroughinhibitingerk12andactivatingppargbothinvitroandinvivo
AT chunmeili persimmontanninpromotedmacrophagereversecholesteroltransportthroughinhibitingerk12andactivatingppargbothinvitroandinvivo
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