Pre-culture Sudan Black B treatment suppresses autofluorescence signals emitted from polymer tissue scaffolds

Abstract In tissue engineering, autofluorescence of polymer scaffolds often lowers the image contrast, making it difficult to examine cells and subcellular structures. Treating the scaffold materials with Sudan Black B (SBB) after cell fixation can effectively suppress autofluorescence, but this app...

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Bibliographic Details
Main Authors: Lin Qi, Erin K. Knapton, Xu Zhang, Tongwen Zhang, Chen Gu, Yi Zhao
Format: Article
Language:English
Published: Nature Publishing Group 2017-08-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-017-08723-2