Use of the polymerase chain reaction for detection of Fusarium graminearum in bulgur wheat
The detection of mycotoxigenic fungi in foodstuff is important because their presence may indicate the possible associated mycotoxin contamination. Fusarium graminearum is a wheat pathogen and a producer of micotoxins. The polymerase chain reaction (PCR) has been employed for the specific identifica...
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Sociedade Brasileira de Ciência e Tecnologia de Alimentos
2012-03-01
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doaj-026c1188fa11460483fc7ec4f98682652020-11-24T22:53:18ZengSociedade Brasileira de Ciência e Tecnologia de AlimentosFood Science and Technology1678-457X2012-03-01321201208S0101-20612012000100030Use of the polymerase chain reaction for detection of Fusarium graminearum in bulgur wheatCarla Bertechini Faria0Giovana Caputo Almeida-Ferreira1Karina Bertechine Gagliardi2Tatiane Cristina Albuquerque Alves3Dauri José Tessmann4Miguel Machinski Junior5Ione Parra Barbosa-Tessmann6Universidade Estadual de MaringáCentro Universitário CesumarUniversidade Estadual de MaringáUniversidade Estadual de MaringáUniversidade Estadual de MaringáUniversidade Estadual de MaringáUniversidade Estadual de MaringáThe detection of mycotoxigenic fungi in foodstuff is important because their presence may indicate the possible associated mycotoxin contamination. Fusarium graminearum is a wheat pathogen and a producer of micotoxins. The polymerase chain reaction (PCR) has been employed for the specific identification of F. graminearum. However, this methodology has not been commonly used for detection of F. graminearum in food. Thus, the objective of the present study was to develop a molecular methodology to detect F. graminearum in commercial samples of bulgur wheat. Two methods were tested. In the first method, a sample of this cereal was contaminated with F. graminearum mycelia. The genomic DNA was extracted from this mixture and used in a F. graminearum specific PCR reaction. The F. graminearum species was detected only in samples that were heavily contaminated. In the second method, samples of bulgur wheat were inoculated on a solid medium, and isolates having F. graminearum culture characteristics were obtained. The DNA extracted from these isolates was tested in F. graminearum specific PCR reactions. An isolate obtained had its trichothecene genotype identified by PCR. The established methodology could be used in surveys of food contamination with F. graminearum.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000100030&lng=en&tlng=entrigo para quibeFusarium graminearumPCRdetecção molecularproteção de alimentos |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Carla Bertechini Faria Giovana Caputo Almeida-Ferreira Karina Bertechine Gagliardi Tatiane Cristina Albuquerque Alves Dauri José Tessmann Miguel Machinski Junior Ione Parra Barbosa-Tessmann |
spellingShingle |
Carla Bertechini Faria Giovana Caputo Almeida-Ferreira Karina Bertechine Gagliardi Tatiane Cristina Albuquerque Alves Dauri José Tessmann Miguel Machinski Junior Ione Parra Barbosa-Tessmann Use of the polymerase chain reaction for detection of Fusarium graminearum in bulgur wheat Food Science and Technology trigo para quibe Fusarium graminearum PCR detecção molecular proteção de alimentos |
author_facet |
Carla Bertechini Faria Giovana Caputo Almeida-Ferreira Karina Bertechine Gagliardi Tatiane Cristina Albuquerque Alves Dauri José Tessmann Miguel Machinski Junior Ione Parra Barbosa-Tessmann |
author_sort |
Carla Bertechini Faria |
title |
Use of the polymerase chain reaction for detection of Fusarium graminearum in bulgur wheat |
title_short |
Use of the polymerase chain reaction for detection of Fusarium graminearum in bulgur wheat |
title_full |
Use of the polymerase chain reaction for detection of Fusarium graminearum in bulgur wheat |
title_fullStr |
Use of the polymerase chain reaction for detection of Fusarium graminearum in bulgur wheat |
title_full_unstemmed |
Use of the polymerase chain reaction for detection of Fusarium graminearum in bulgur wheat |
title_sort |
use of the polymerase chain reaction for detection of fusarium graminearum in bulgur wheat |
publisher |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
series |
Food Science and Technology |
issn |
1678-457X |
publishDate |
2012-03-01 |
description |
The detection of mycotoxigenic fungi in foodstuff is important because their presence may indicate the possible associated mycotoxin contamination. Fusarium graminearum is a wheat pathogen and a producer of micotoxins. The polymerase chain reaction (PCR) has been employed for the specific identification of F. graminearum. However, this methodology has not been commonly used for detection of F. graminearum in food. Thus, the objective of the present study was to develop a molecular methodology to detect F. graminearum in commercial samples of bulgur wheat. Two methods were tested. In the first method, a sample of this cereal was contaminated with F. graminearum mycelia. The genomic DNA was extracted from this mixture and used in a F. graminearum specific PCR reaction. The F. graminearum species was detected only in samples that were heavily contaminated. In the second method, samples of bulgur wheat were inoculated on a solid medium, and isolates having F. graminearum culture characteristics were obtained. The DNA extracted from these isolates was tested in F. graminearum specific PCR reactions. An isolate obtained had its trichothecene genotype identified by PCR. The established methodology could be used in surveys of food contamination with F. graminearum. |
topic |
trigo para quibe Fusarium graminearum PCR detecção molecular proteção de alimentos |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000100030&lng=en&tlng=en |
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