Sorghum polyphenol suppresses the growth as well as metastasis of colon cancer xenografts through co-targeting jak2/STAT3 and PI3K/Akt/mTOR pathways
Sorghum contains phytochemicals that can protect against multiple forms of cancers. However, the underlying molecular targets and cellular mechanisms remain unknown. We investigated the role of Hwanggeumchal sorghum extracts (HSE) on colon cancer cells. We found HSE inhibited the proliferation of hu...
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doaj-0273f4b18dbb423ea0be412913fbfe732021-04-29T04:44:08ZengElsevierJournal of Functional Foods1756-46462015-05-0115193206Sorghum polyphenol suppresses the growth as well as metastasis of colon cancer xenografts through co-targeting jak2/STAT3 and PI3K/Akt/mTOR pathwaysPramod Darvin0Youn Hee Joung1Nipin S P2Dong Young Kang3Hyo Joo Byun4Dae Yong Hwang5Kwang Hyun Cho6Kyung Do Park7Hak Kyo Lee8Young Mok Yang9Department of Pathology, School of Medicine, Institute of Biomedical Science and Technology, Konkuk University Glocal Campus, Seoul 143-701, Republic of KoreaDepartment of Pathology, School of Medicine, Institute of Biomedical Science and Technology, Konkuk University Glocal Campus, Seoul 143-701, Republic of KoreaDepartment of Pathology, School of Medicine, Institute of Biomedical Science and Technology, Konkuk University Glocal Campus, Seoul 143-701, Republic of KoreaDepartment of Pathology, School of Medicine, Institute of Biomedical Science and Technology, Konkuk University Glocal Campus, Seoul 143-701, Republic of KoreaDepartment of Pathology, School of Medicine, Institute of Biomedical Science and Technology, Konkuk University Glocal Campus, Seoul 143-701, Republic of KoreaDepartment of Surgery, School of Medicine, Konkuk University, Seoul 143-701, Republic of KoreaNational Institute of Animal Science, RDA, Cheonan 330-800, Republic of KoreaGenomics Informatics Center, Hankyong National University, Anseong 456-749, Republic of KoreaGenomics Informatics Center, Hankyong National University, Anseong 456-749, Republic of Korea; Corresponding author. Genomics Informatics Center, Hankyong National University, Anseong 456-749, Republic of Korea. Tel.: +82316705332; fax: +82316705339.Department of Pathology, School of Medicine, Institute of Biomedical Science and Technology, Konkuk University Glocal Campus, Seoul 143-701, Republic of Korea; Corresponding author. Department of Pathology, School of Medicine, and Institute of Biomedical Science and Technology, Konkuk University, Seoul 143-701, Republic of Korea. Tel.: +82220307812; fax: +82220307845.Sorghum contains phytochemicals that can protect against multiple forms of cancers. However, the underlying molecular targets and cellular mechanisms remain unknown. We investigated the role of Hwanggeumchal sorghum extracts (HSE) on colon cancer cells. We found HSE inhibited the proliferation of human colon cancer cells in a dose-dependent manner by inducing G1 phase arrest and apoptosis. Likewise, it suppressed the Jak2/STAT3 and PI3K/AKT/mTOR pathways. Studies on STAT3 transcription regulatory functions showed HSE inhibited the STAT3/DNA binding and transcription promoter activity. Thereby the expression of STAT3 downstream targets VEGF and VEGF-R2 were dropped. Furthermore, HSE treatment suppressed tumor growth and pulmonary metastasis in animal models. Together, these findings suggested that the targeted inhibition of Jak2/STAT3 and PI3K/Akt/mTOR contribute to cancer therapy potential of H. sorghum (HS). Thus, use of sorghum as a dietary supplement has the potential as a nutraceutical to prevent the onset of colon cancer, without any side effects.http://www.sciencedirect.com/science/article/pii/S1756464615001280Hwanggeumchal sorghumJak2/STAT3PI3K/Akt/mTORColon cancer xenograftsPulmonary metastasis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pramod Darvin Youn Hee Joung Nipin S P Dong Young Kang Hyo Joo Byun Dae Yong Hwang Kwang Hyun Cho Kyung Do Park Hak Kyo Lee Young Mok Yang |
spellingShingle |
Pramod Darvin Youn Hee Joung Nipin S P Dong Young Kang Hyo Joo Byun Dae Yong Hwang Kwang Hyun Cho Kyung Do Park Hak Kyo Lee Young Mok Yang Sorghum polyphenol suppresses the growth as well as metastasis of colon cancer xenografts through co-targeting jak2/STAT3 and PI3K/Akt/mTOR pathways Journal of Functional Foods Hwanggeumchal sorghum Jak2/STAT3 PI3K/Akt/mTOR Colon cancer xenografts Pulmonary metastasis |
author_facet |
Pramod Darvin Youn Hee Joung Nipin S P Dong Young Kang Hyo Joo Byun Dae Yong Hwang Kwang Hyun Cho Kyung Do Park Hak Kyo Lee Young Mok Yang |
author_sort |
Pramod Darvin |
title |
Sorghum polyphenol suppresses the growth as well as metastasis of colon cancer xenografts through co-targeting jak2/STAT3 and PI3K/Akt/mTOR pathways |
title_short |
Sorghum polyphenol suppresses the growth as well as metastasis of colon cancer xenografts through co-targeting jak2/STAT3 and PI3K/Akt/mTOR pathways |
title_full |
Sorghum polyphenol suppresses the growth as well as metastasis of colon cancer xenografts through co-targeting jak2/STAT3 and PI3K/Akt/mTOR pathways |
title_fullStr |
Sorghum polyphenol suppresses the growth as well as metastasis of colon cancer xenografts through co-targeting jak2/STAT3 and PI3K/Akt/mTOR pathways |
title_full_unstemmed |
Sorghum polyphenol suppresses the growth as well as metastasis of colon cancer xenografts through co-targeting jak2/STAT3 and PI3K/Akt/mTOR pathways |
title_sort |
sorghum polyphenol suppresses the growth as well as metastasis of colon cancer xenografts through co-targeting jak2/stat3 and pi3k/akt/mtor pathways |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2015-05-01 |
description |
Sorghum contains phytochemicals that can protect against multiple forms of cancers. However, the underlying molecular targets and cellular mechanisms remain unknown. We investigated the role of Hwanggeumchal sorghum extracts (HSE) on colon cancer cells. We found HSE inhibited the proliferation of human colon cancer cells in a dose-dependent manner by inducing G1 phase arrest and apoptosis. Likewise, it suppressed the Jak2/STAT3 and PI3K/AKT/mTOR pathways. Studies on STAT3 transcription regulatory functions showed HSE inhibited the STAT3/DNA binding and transcription promoter activity. Thereby the expression of STAT3 downstream targets VEGF and VEGF-R2 were dropped. Furthermore, HSE treatment suppressed tumor growth and pulmonary metastasis in animal models. Together, these findings suggested that the targeted inhibition of Jak2/STAT3 and PI3K/Akt/mTOR contribute to cancer therapy potential of H. sorghum (HS). Thus, use of sorghum as a dietary supplement has the potential as a nutraceutical to prevent the onset of colon cancer, without any side effects. |
topic |
Hwanggeumchal sorghum Jak2/STAT3 PI3K/Akt/mTOR Colon cancer xenografts Pulmonary metastasis |
url |
http://www.sciencedirect.com/science/article/pii/S1756464615001280 |
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