LXR activation by GW3965 alters fat tissue distribution and adipose tissue inflammation in ob/ob female mice[S]
To investigate the role of liver X receptor (LXR) in adipose tissue metabolism during obesity, ob/ob mice were treated for 5 weeks with the synthetic LXR agonist GW3965. MRI analysis revealed that pharmacological activation of LXR modified fat distribution by decreasing visceral (VS) fat and inverse...
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doaj-027985524ccf408697705e3b70a0de822021-04-28T06:05:55ZengElsevierJournal of Lipid Research0022-22752013-05-0154513001311LXR activation by GW3965 alters fat tissue distribution and adipose tissue inflammation in ob/ob female mice[S]Amena Archer0Émilie Stolarczyk1Maria Luisa Doria2Luisa Helguero3Rosário Domingues4Jane K. Howard5Agneta Mode6Marion Korach-André7Jan-Åke Gustafsson8To whom correspondence should be addressed.;amena.archer@ki.se amena.archer@gmail.com; Department of Biosciences and Nutrition, Karolinska Insitutet, Huddinge, Sweden, TX; To whom correspondence should be addressed.;amena.archer@ki.se amena.archer@gmail.comDivision of Diabetes and Nutritional Sciences, King舗s College London, London, United Kingdom, TXDepartment of Organic Chemistry and Natural Products, Universidade de Aveiro, Aveiro, Portugal, TXDepartment of Organic Chemistry and Natural Products, Universidade de Aveiro, Aveiro, Portugal, TXDepartment of Organic Chemistry and Natural Products, Universidade de Aveiro, Aveiro, Portugal, TXDivision of Diabetes and Nutritional Sciences, King舗s College London, London, United Kingdom, TXDepartment of Biosciences and Nutrition, Karolinska Insitutet, Huddinge, Sweden, TXDepartment of Biosciences and Nutrition, Karolinska Insitutet, Huddinge, Sweden, TXDepartment of Biosciences and Nutrition, Karolinska Insitutet, Huddinge, Sweden, TX; Department of Biology and Biochemistry, Center for Nuclear Receptors and Cell Signaling, University of Houston, Houston, TXTo investigate the role of liver X receptor (LXR) in adipose tissue metabolism during obesity, ob/ob mice were treated for 5 weeks with the synthetic LXR agonist GW3965. MRI analysis revealed that pharmacological activation of LXR modified fat distribution by decreasing visceral (VS) fat and inversely increasing subcutaneous (SC) fat storage without affecting whole body fat content. This was concordant with opposite regulation by GW3965 of the lipolytic markers hormone-sensitive lipase (HSL) and adipose triglyceride lipase (ATGL) in the two fat depots; moreover, the expression of genes involved in lipogenesis was significantly induced in SC fat. Lipidomic analysis suggested that changes in lipid composition in response to GW3965 also varied between VS and SC fat. In both depots, the observed alteration in lipid composition indicated an overall change toward less lipotoxic lipids. Flow cytometry analysis showed decreased immune cell infiltration in adipose tissue of ob/ob mice in response to GW3965 treatment, which in VS fat mainly affected the macrophage population and in SC fat the lymphocyte population. In line with this, the expression and secretion of proinflammatory markers was decreased in both fat deposits with GW3965 treatment.http://www.sciencedirect.com/science/article/pii/S0022227520421686liver X receptorlipidomic analysisimmune cell infiltration |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Amena Archer Émilie Stolarczyk Maria Luisa Doria Luisa Helguero Rosário Domingues Jane K. Howard Agneta Mode Marion Korach-André Jan-Åke Gustafsson |
spellingShingle |
Amena Archer Émilie Stolarczyk Maria Luisa Doria Luisa Helguero Rosário Domingues Jane K. Howard Agneta Mode Marion Korach-André Jan-Åke Gustafsson LXR activation by GW3965 alters fat tissue distribution and adipose tissue inflammation in ob/ob female mice[S] Journal of Lipid Research liver X receptor lipidomic analysis immune cell infiltration |
author_facet |
Amena Archer Émilie Stolarczyk Maria Luisa Doria Luisa Helguero Rosário Domingues Jane K. Howard Agneta Mode Marion Korach-André Jan-Åke Gustafsson |
author_sort |
Amena Archer |
title |
LXR activation by GW3965 alters fat tissue distribution and adipose tissue inflammation in ob/ob female mice[S] |
title_short |
LXR activation by GW3965 alters fat tissue distribution and adipose tissue inflammation in ob/ob female mice[S] |
title_full |
LXR activation by GW3965 alters fat tissue distribution and adipose tissue inflammation in ob/ob female mice[S] |
title_fullStr |
LXR activation by GW3965 alters fat tissue distribution and adipose tissue inflammation in ob/ob female mice[S] |
title_full_unstemmed |
LXR activation by GW3965 alters fat tissue distribution and adipose tissue inflammation in ob/ob female mice[S] |
title_sort |
lxr activation by gw3965 alters fat tissue distribution and adipose tissue inflammation in ob/ob female mice[s] |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2013-05-01 |
description |
To investigate the role of liver X receptor (LXR) in adipose tissue metabolism during obesity, ob/ob mice were treated for 5 weeks with the synthetic LXR agonist GW3965. MRI analysis revealed that pharmacological activation of LXR modified fat distribution by decreasing visceral (VS) fat and inversely increasing subcutaneous (SC) fat storage without affecting whole body fat content. This was concordant with opposite regulation by GW3965 of the lipolytic markers hormone-sensitive lipase (HSL) and adipose triglyceride lipase (ATGL) in the two fat depots; moreover, the expression of genes involved in lipogenesis was significantly induced in SC fat. Lipidomic analysis suggested that changes in lipid composition in response to GW3965 also varied between VS and SC fat. In both depots, the observed alteration in lipid composition indicated an overall change toward less lipotoxic lipids. Flow cytometry analysis showed decreased immune cell infiltration in adipose tissue of ob/ob mice in response to GW3965 treatment, which in VS fat mainly affected the macrophage population and in SC fat the lymphocyte population. In line with this, the expression and secretion of proinflammatory markers was decreased in both fat deposits with GW3965 treatment. |
topic |
liver X receptor lipidomic analysis immune cell infiltration |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520421686 |
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