Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana
Abstract Background CRISPR-Cas is a recent and powerful addition to the molecular toolbox which allows programmable genome editing. It has been used to modify genes in a wide variety of organisms, but only two alga to date. Here we present a methodology to edit the genome of Thalassiosira pseudonana...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2016-11-01
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| Series: | Plant Methods |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13007-016-0148-0 |