Expression of Novel L-Leucine Dehydrogenase and High-Level Production of L-Tert-Leucine Catalyzed by Engineered Escherichia coli

Expression of Novel L-Leucine Dehydrogenase and High-Level Production of L-Tert-Leucine Catalyzed by Engineered Escherichia coli

Leucine dehydrogenase (LDH) is a NAD+-dependent oxidoreductase, which can selectively catalyze α-keto acids to obtain α-amino acids and their derivatives. It plays a key role in the biosynthesis of L-tert-leucine (L-Tle). As a non-naturally chiral amino acid, L-Tle can be used as an animal feed addi...

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Bibliographic Details
Main Authors: Yuan-Yuan Jia, Yu-Li Xie, Lu-Lu Yang, Hong-Ling Shi, Yun-Feng Lu, Si-Pu Zhang, Cun-Duo Tang, Lun-Guang Yao, Yun-Chao Kan
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-03-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
leucine dehydrogenase
feed additive
expression
biocatalysis
formate dehydrogenase
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2021.655522/full

Internet

https://www.frontiersin.org/articles/10.3389/fbioe.2021.655522/full

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