A Comparative View on Human Somatic Cell Sources for iPSC Generation
The breakthrough of reprogramming human somatic cells was achieved in 2006 by the work of Yamanaka and Takahashi. From this point, fibroblasts are the most commonly used primary somatic cell type for the generation of induced pluripotent stem cells (iPSCs). Various characteristics of fibroblasts sup...
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Online Access: | http://dx.doi.org/10.1155/2014/768391 |
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doaj-03b87caeceab4528a344ed2e35a530422020-11-24T23:20:24ZengHindawi LimitedStem Cells International1687-966X1687-96782014-01-01201410.1155/2014/768391768391A Comparative View on Human Somatic Cell Sources for iPSC GenerationStefanie Raab0Moritz Klingenstein1Stefan Liebau2Leonhard Linta3Institute of Neuroanatomy, Eberhard Karls University Tübingen, Österbergstraße 3, 72074 Tübingen, GermanyInstitute of Neuroanatomy, Eberhard Karls University Tübingen, Österbergstraße 3, 72074 Tübingen, GermanyInstitute of Neuroanatomy, Eberhard Karls University Tübingen, Österbergstraße 3, 72074 Tübingen, GermanyInstitute of Neuroanatomy, Eberhard Karls University Tübingen, Österbergstraße 3, 72074 Tübingen, GermanyThe breakthrough of reprogramming human somatic cells was achieved in 2006 by the work of Yamanaka and Takahashi. From this point, fibroblasts are the most commonly used primary somatic cell type for the generation of induced pluripotent stem cells (iPSCs). Various characteristics of fibroblasts supported their utilization for the groundbreaking experiments of iPSC generation. One major advantage is the high availability of fibroblasts which can be easily isolated from skin biopsies. Furthermore, their cultivation, propagation, and cryoconservation properties are uncomplicated with respect to nutritional requirements and viability in culture. However, the required skin biopsy remains an invasive approach, representing a major drawback for using fibroblasts as the starting material. More and more studies appeared over the last years, describing the reprogramming of other human somatic cell types. Cells isolated from blood samples or urine, as well as more unexpected cell types, like pancreatic islet beta cells, synovial cells, or mesenchymal stromal cells from wisdom teeth, show promising characteristics for a reprogramming strategy. Here, we want to highlight the advantages of keratinocytes from human plucked hair as a widely usable, noninvasive harvesting method for primary material in comparison with other commonly used cell types.http://dx.doi.org/10.1155/2014/768391 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Stefanie Raab Moritz Klingenstein Stefan Liebau Leonhard Linta |
spellingShingle |
Stefanie Raab Moritz Klingenstein Stefan Liebau Leonhard Linta A Comparative View on Human Somatic Cell Sources for iPSC Generation Stem Cells International |
author_facet |
Stefanie Raab Moritz Klingenstein Stefan Liebau Leonhard Linta |
author_sort |
Stefanie Raab |
title |
A Comparative View on Human Somatic Cell Sources for iPSC Generation |
title_short |
A Comparative View on Human Somatic Cell Sources for iPSC Generation |
title_full |
A Comparative View on Human Somatic Cell Sources for iPSC Generation |
title_fullStr |
A Comparative View on Human Somatic Cell Sources for iPSC Generation |
title_full_unstemmed |
A Comparative View on Human Somatic Cell Sources for iPSC Generation |
title_sort |
comparative view on human somatic cell sources for ipsc generation |
publisher |
Hindawi Limited |
series |
Stem Cells International |
issn |
1687-966X 1687-9678 |
publishDate |
2014-01-01 |
description |
The breakthrough of reprogramming human somatic cells was achieved in 2006 by the work of Yamanaka and Takahashi. From this point, fibroblasts are the most commonly used primary somatic cell type for the generation of induced pluripotent stem cells (iPSCs). Various characteristics of fibroblasts supported their utilization for the groundbreaking experiments of iPSC generation. One major advantage is the high availability of fibroblasts which can be easily isolated from skin biopsies. Furthermore, their cultivation, propagation, and cryoconservation properties are uncomplicated with respect to nutritional requirements and viability in culture. However, the required skin biopsy remains an invasive approach, representing a major drawback for using fibroblasts as the starting material. More and more studies appeared over the last years, describing the reprogramming of other human somatic cell types. Cells isolated from blood samples or urine, as well as more unexpected cell types, like pancreatic islet beta cells, synovial cells, or mesenchymal stromal cells from wisdom teeth, show promising characteristics for a reprogramming strategy. Here, we want to highlight the advantages of keratinocytes from human plucked hair as a widely usable, noninvasive harvesting method for primary material in comparison with other commonly used cell types. |
url |
http://dx.doi.org/10.1155/2014/768391 |
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