Effects of mRNA secondary structure on the expression of HEV ORF2 proteins in Escherichia coli

Effects of mRNA secondary structure on the expression of HEV ORF2 proteins in Escherichia coli

Abstract Background Viral protein expression in Escherichia coli (E. coli) is a powerful tool for structural/functional studies as well as for vaccine and diagnostics development. However, numerous factors such as codon bias, mRNA secondary structure and nucleotides distribution, have been indentifi...

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Bibliographic Details
Main Authors: Nouredine Behloul, Wenjuan Wei, Sarra Baha, Zhenzhen Liu, Jiyue Wen, Jihong Meng
Format: Article
Language:English
Published: BMC 2017-11-01
Series:Microbial Cell Factories
Subjects:
Recombinant protein expression
Escherichia coli
RNA secondary structure
Hepatitis E virus
Online Access:http://link.springer.com/article/10.1186/s12934-017-0812-8

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http://link.springer.com/article/10.1186/s12934-017-0812-8

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