Novel degenerate PCR method for whole genome amplification applied to Peru Margin (ODP Leg 201) subsurface samples

Novel degenerate PCR method for whole genome amplification applied to Peru Margin (ODP Leg 201) subsurface samples

A degenerate PCR-based method of whole-genome amplification, designed to work fluidly with 454 sequencing technology, was developed and tested for use on deep marine subsurface DNA samples. The method, which we have called Random Amplification Metagenomic PCR (RAMP), involves the use of specific pr...

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Bibliographic Details
Main Authors: Amanda eMartino, Matthew E. Rhodes, Jennifer F Biddle, Leah D. Brandt, Lynn P. Tomsho, Christopher H House
Format: Article
Language:English
Published: Frontiers Media S.A. 2012-01-01
Series:Frontiers in Microbiology
Subjects:
Archaea
Bacteria
Metagenomics
deep biosphere
ocean drilling
low-biomass
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2012.00017/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fmicb.2012.00017/full

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