Defined tetra-allelic gene disruption of the 4-coumarate:coenzyme A ligase 1 (Pv4CL1) gene by CRISPR/Cas9 in switchgrass results in lignin reduction and improved sugar release

Defined tetra-allelic gene disruption of the 4-coumarate:coenzyme A ligase 1 (Pv4CL1) gene by CRISPR/Cas9 in switchgrass results in lignin reduction and improved sugar release

Abstract Background The development of genome editing technologies offers new prospects in improving bioenergy crops like switchgrass (Panicum virgatum). Switchgrass is an outcrossing species with an allotetraploid genome (2n = 4x = 36), a complexity which forms an impediment to generating homozygou...

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Bibliographic Details
Main Authors: Jong-Jin Park, Chang Geun Yoo, Amy Flanagan, Yunqiao Pu, Smriti Debnath, Yaxin Ge, Arthur J. Ragauskas, Zeng-Yu Wang
Format: Article
Language:English
Published: BMC 2017-11-01
Series:Biotechnology for Biofuels
Subjects:
Genome editing
CRISPR/Cas9
Switchgrass
Panicum virgatum
Bioenergy
Lignin biosynthesis
Online Access:http://link.springer.com/article/10.1186/s13068-017-0972-0

Internet

http://link.springer.com/article/10.1186/s13068-017-0972-0

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