Fragmentation of Pooled PCR Products for Highly Multiplexed TILLING

Fragmentation of Pooled PCR Products for Highly Multiplexed TILLING

Improvements to massively parallel sequencing have allowed the routine recovery of natural and induced sequence variants. A broad range of biological disciplines have benefited from this, ranging from plant breeding to cancer research. The need for high sequence coverage to accurately recover single...

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Bibliographic Details
Main Authors: Andrea Tramontano, Luka Jarc, Joanna Jankowicz-Cieslak, Bernhard J. Hofinger, Katarzyna Gajek, Miriam Szurman-Zubrzycka, Iwona Szarejko, Ivan Ingelbrecht, Bradley J. Till
Format: Article
Language:English
Published: Oxford University Press 2019-08-01
Series:G3: Genes, Genomes, Genetics
Subjects:
TILLING by Sequencing
DNA shearing
sonication
Hordeum vulgare
barley
Online Access:http://g3journal.org/lookup/doi/10.1534/g3.119.400301

Internet

http://g3journal.org/lookup/doi/10.1534/g3.119.400301

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