Utilization of Digital PCR in Quantity Verification of Plasmid Standards Used in Quantitative PCR

Utilization of Digital PCR in Quantity Verification of Plasmid Standards Used in Quantitative PCR

Quantitative PCR (qPCR) is a widely used method for nucleic acid quantification of various pathogenic microorganisms. For absolute quantification of microbial load by qPCR, it is essential to create a calibration curve from accurately quantified quantification standards, from which the number of pat...

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Bibliographic Details
Main Authors: Martina Beinhauerova, Vladimir Babak, Barbara Bertasi, Maria Beatrice Boniotti, Petr Kralik
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-07-01
Series:Frontiers in Molecular Biosciences
Subjects:
digital PCR
absolute quantification
quantity verification
quantification plasmid standard
qPCR
real time PCR
Online Access:https://www.frontiersin.org/article/10.3389/fmolb.2020.00155/full

Internet

https://www.frontiersin.org/article/10.3389/fmolb.2020.00155/full

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