Subsampled open-reference clustering creates consistent, comprehensive OTU definitions and scales to billions of sequences

Subsampled open-reference clustering creates consistent, comprehensive OTU definitions and scales to billions of sequences

We present a performance-optimized algorithm, subsampled open-reference OTU picking, for assigning marker gene (e.g., 16S rRNA) sequences generated on next-generation sequencing platforms to operational taxonomic units (OTUs) for microbial community analysis. This algorithm provides benefits over de...

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Bibliographic Details
Main Authors: Jai Ram Rideout, Yan He, Jose A. Navas-Molina, William A. Walters, Luke K. Ursell, Sean M. Gibbons, John Chase, Daniel McDonald, Antonio Gonzalez, Adam Robbins-Pianka, Jose C. Clemente, Jack A. Gilbert, Susan M. Huse, Hong-Wei Zhou, Rob Knight, J. Gregory Caporaso
Format: Article
Language:English
Published: PeerJ Inc. 2014-08-01
Series:PeerJ
Subjects:
OTU picking
Microbial ecology
Microbiome
Qiime
Bioinformatics
Online Access:https://peerj.com/articles/545.pdf

Internet

https://peerj.com/articles/545.pdf

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