Quantitative isolation of total glycosphingolipids from animal cells

The quantitative isolation of total glycosphingolipids from crude lipid extracts without contamination from other lipid classes is described. The method consists of (a) acetylation of total lipids with pyridine and acetic anhydride, (b) separation of acetylated glycolipids from nonglycolipids on a m...

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Bibliographic Details
Main Authors: Terunobu Saito, Sen-itiroh Hakomori
Format: Article
Language:English
Published: Elsevier 1971-03-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520395377