Expansion of targetable sites for the ribonucleoprotein-based CRISPR/Cas9 system in the silkworm Bombyx mori

Expansion of targetable sites for the ribonucleoprotein-based CRISPR/Cas9 system in the silkworm Bombyx mori

Abstract Background With the emergence of CRISPR/Cas9 technology, multiple gene editing procedures became available for the silkworm. Although binary transgene-based methods have been widely used to generate mutants, delivery of the CRISPR/Cas9 system via DNA-free ribonucleoproteins offers several a...

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Bibliographic Details
Main Authors: Yun-long Zou, Ai-jun Ye, Shuo Liu, Wen-tao Wu, Li-feng Xu, Fang-yin Dai, Xiao-ling Tong
Format: Article
Language:English
Published: BMC 2021-09-01
Series:BMC Biotechnology
Subjects:
CRISPR/Cas9
Ribonucleoprotein
Silkworm
Target repertoire
BmBLOS2
BmGR66
Online Access:https://doi.org/10.1186/s12896-021-00714-6

Internet

https://doi.org/10.1186/s12896-021-00714-6

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