Expansion of targetable sites for the ribonucleoprotein-based CRISPR/Cas9 system in the silkworm Bombyx mori
Abstract Background With the emergence of CRISPR/Cas9 technology, multiple gene editing procedures became available for the silkworm. Although binary transgene-based methods have been widely used to generate mutants, delivery of the CRISPR/Cas9 system via DNA-free ribonucleoproteins offers several a...
Main Authors: | Yun-long Zou, Ai-jun Ye, Shuo Liu, Wen-tao Wu, Li-feng Xu, Fang-yin Dai, Xiao-ling Tong |
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Format: | Article |
Language: | English |
Published: |
BMC
2021-09-01
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Series: | BMC Biotechnology |
Subjects: | |
Online Access: | https://doi.org/10.1186/s12896-021-00714-6 |
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