One-Tube-Only Standardized Site-Directed Mutagenesis: An Alternative Approach to Generate Amino Acid Substitution Collections.

One-Tube-Only Standardized Site-Directed Mutagenesis: An Alternative Approach to Generate Amino Acid Substitution Collections.

Site-directed mutagenesis (SDM) is a powerful tool to create defined collections of protein variants for experimental and clinical purposes, but effectiveness is compromised when a large number of mutations is required. We present here a one-tube-only standardized SDM approach that generates compreh...

Full description

Bibliographic Details
Main Authors: Janire Mingo, Asier Erramuzpe, Sandra Luna, Olaia Aurtenetxe, Laura Amo, Ibai Diez, Jan T G Schepens, Wiljan J A J Hendriks, Jesús M Cortés, Rafael Pulido
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4993582?pdf=render

Internet

http://europepmc.org/articles/PMC4993582?pdf=render

Similar Items