A High-Throughput, Precipitating Colorimetric Sandwich ELISA Microarray for Shiga Toxins

A High-Throughput, Precipitating Colorimetric Sandwich ELISA Microarray for Shiga Toxins

Shiga toxins 1 and 2 (Stx1 and Stx2) from Shiga toxin-producing E. coli (STEC) bacteria were simultaneously detected with a newly developed, high-throughput antibody microarray platform. The proteinaceous toxins were immobilized and sandwiched between biorecognition elements (monoclonal antibodies)...

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Bibliographic Details
Main Authors: Andrew Gehring, Xiaohua He, Pina Fratamico, Joseph Lee, Lori Bagi, Jeffrey Brewster, George Paoli, Yiping He, Yanping Xie, Craig Skinner, Charlie Barnett, Douglas Harris
Format: Article
Language:English
Published: MDPI AG 2014-06-01
Series:Toxins
Subjects:
B-PER
colorimetry
detection
ELISA
high-throughput
microarray STEC
microtiter plate
precipitating
toxin typing
Online Access:http://www.mdpi.com/2072-6651/6/6/1855

Internet

http://www.mdpi.com/2072-6651/6/6/1855

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