Involvement of atypical protein kinase C in the regulation of cardiac glucose and long-chain fatty acid uptake
Aim: The signaling pathways involved in the regulation of cardiac GLUT4 translocation/glucose uptake and CD36 translocation/ long-chain fatty acid uptake are not fully understood. We compared in heart/muscle-specific PKC-λ knockout mice the roles of atypical PKCs (PKC-ζ and PKC-λ) in regulating card...
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doaj-04f8238d65434a8581d9b4b612769d9e2020-11-24T23:19:37ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2012-09-01310.3389/fphys.2012.0036121733Involvement of atypical protein kinase C in the regulation of cardiac glucose and long-chain fatty acid uptakeDaphna D.J. Habets0Joost J.F.P. Luiken1Margriet eOuwens2Will A. Coumans3Monique eVergouwe4Stine J. Maarbjerg5Michael eLeitges6Arend eBonen7Erik A. Richter8Jan eGlatz9Maastricht UniversityMaastricht UniversityGerman Diabetes CenterMaastricht UniversityMaastricht UniversityUniversity of CopenhagenBiotechnology Centre of OsloUniversity of GuelphUniversity of CopenhagenMaastricht UniversityAim: The signaling pathways involved in the regulation of cardiac GLUT4 translocation/glucose uptake and CD36 translocation/ long-chain fatty acid uptake are not fully understood. We compared in heart/muscle-specific PKC-λ knockout mice the roles of atypical PKCs (PKC-ζ and PKC-λ) in regulating cardiac glucose and fatty acid uptake. Results: Neither insulin-stimulated nor AMPK-mediated glucose and fatty acid uptake were inhibited upon genetic PKC-λ ablation in cardiomyocytes. In contrast, myristoylated PKC-ζ pseudosubstrate inhibited both insulin-stimulated and AMPK-mediated glucose and fatty acid uptake by >80% in both wild-type and PKC-λ-knockout cardiomyocytes. In PKC-λ knockout cardiomyocytes, PKC-ζ is the sole remaining atypical PKC isoform, and its expression level is not different from wild-type cardiomyocytes, in which it contributes to 29% and 17% of total atypical PKC expression and phosphorylation, respectively. Conclusion: Taken together, atypical PKCs are necessary for insulin-stimulated and AMPK-mediated glucose uptake into the heart, as well as for insulin-stimulated and AMPK-mediated fatty acid uptake. However, the residual PKC-ζ activity in PKC-λ-knockout cardiomyocytes is sufficient to allow optimal stimulation of glucose and fatty acid uptake, indicating that atypical PKCs are necessary but not rate-limiting in the regulation of cardiac substrate uptake and that PKC-λ and PKC-ζ have interchangeable functions in these processes.http://journal.frontiersin.org/Journal/10.3389/fphys.2012.00361/fullInsulincardiomyocytesAMPKCD36atypical PKCfatty acid uptake |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Daphna D.J. Habets Joost J.F.P. Luiken Margriet eOuwens Will A. Coumans Monique eVergouwe Stine J. Maarbjerg Michael eLeitges Arend eBonen Erik A. Richter Jan eGlatz |
spellingShingle |
Daphna D.J. Habets Joost J.F.P. Luiken Margriet eOuwens Will A. Coumans Monique eVergouwe Stine J. Maarbjerg Michael eLeitges Arend eBonen Erik A. Richter Jan eGlatz Involvement of atypical protein kinase C in the regulation of cardiac glucose and long-chain fatty acid uptake Frontiers in Physiology Insulin cardiomyocytes AMPK CD36 atypical PKC fatty acid uptake |
author_facet |
Daphna D.J. Habets Joost J.F.P. Luiken Margriet eOuwens Will A. Coumans Monique eVergouwe Stine J. Maarbjerg Michael eLeitges Arend eBonen Erik A. Richter Jan eGlatz |
author_sort |
Daphna D.J. Habets |
title |
Involvement of atypical protein kinase C in the regulation of cardiac glucose and long-chain fatty acid uptake |
title_short |
Involvement of atypical protein kinase C in the regulation of cardiac glucose and long-chain fatty acid uptake |
title_full |
Involvement of atypical protein kinase C in the regulation of cardiac glucose and long-chain fatty acid uptake |
title_fullStr |
Involvement of atypical protein kinase C in the regulation of cardiac glucose and long-chain fatty acid uptake |
title_full_unstemmed |
Involvement of atypical protein kinase C in the regulation of cardiac glucose and long-chain fatty acid uptake |
title_sort |
involvement of atypical protein kinase c in the regulation of cardiac glucose and long-chain fatty acid uptake |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Physiology |
issn |
1664-042X |
publishDate |
2012-09-01 |
description |
Aim: The signaling pathways involved in the regulation of cardiac GLUT4 translocation/glucose uptake and CD36 translocation/ long-chain fatty acid uptake are not fully understood. We compared in heart/muscle-specific PKC-λ knockout mice the roles of atypical PKCs (PKC-ζ and PKC-λ) in regulating cardiac glucose and fatty acid uptake. Results: Neither insulin-stimulated nor AMPK-mediated glucose and fatty acid uptake were inhibited upon genetic PKC-λ ablation in cardiomyocytes. In contrast, myristoylated PKC-ζ pseudosubstrate inhibited both insulin-stimulated and AMPK-mediated glucose and fatty acid uptake by >80% in both wild-type and PKC-λ-knockout cardiomyocytes. In PKC-λ knockout cardiomyocytes, PKC-ζ is the sole remaining atypical PKC isoform, and its expression level is not different from wild-type cardiomyocytes, in which it contributes to 29% and 17% of total atypical PKC expression and phosphorylation, respectively. Conclusion: Taken together, atypical PKCs are necessary for insulin-stimulated and AMPK-mediated glucose uptake into the heart, as well as for insulin-stimulated and AMPK-mediated fatty acid uptake. However, the residual PKC-ζ activity in PKC-λ-knockout cardiomyocytes is sufficient to allow optimal stimulation of glucose and fatty acid uptake, indicating that atypical PKCs are necessary but not rate-limiting in the regulation of cardiac substrate uptake and that PKC-λ and PKC-ζ have interchangeable functions in these processes. |
topic |
Insulin cardiomyocytes AMPK CD36 atypical PKC fatty acid uptake |
url |
http://journal.frontiersin.org/Journal/10.3389/fphys.2012.00361/full |
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