Improved Protocol for DNA Extraction from Subsoils Using Phosphate Lysis Buffer

As our understanding of soil biology deepens, there is a growing demand for investigations addressing microbial processes in the earth beneath the topsoil layer, called subsoil. High clay content in subsoils often hinders the recovery of sufficient quantities of DNA as clay particles bind nucleic ac...

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Bibliographic Details
Main Authors: Victor Guerra, Lukas Beule, Ena Lehtsaar, Hui-Ling Liao, Petr Karlovsky
Format: Article
Language:English
Published: MDPI AG 2020-04-01
Series:Microorganisms
Subjects:
Online Access:https://www.mdpi.com/2076-2607/8/4/532