Enhancing Terminal Deoxynucleotidyl Transferase Activity on Substrates with 3′ Terminal Structures for Enzymatic De Novo DNA Synthesis

Enhancing Terminal Deoxynucleotidyl Transferase Activity on Substrates with 3′ Terminal Structures for Enzymatic De Novo DNA Synthesis

Enzymatic oligonucleotide synthesis methods based on the template-independent polymerase terminal deoxynucleotidyl transferase (TdT) promise to enable the de novo synthesis of long oligonucleotides under mild, aqueous conditions. Intermediates with a 3′ terminal structure (hairpins) will i...

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Bibliographic Details
Main Authors: Sebastian Barthel, Sebastian Palluk, Nathan J. Hillson, Jay D. Keasling, Daniel H. Arlow
Format: Article
Language:English
Published: MDPI AG 2020-01-01
Series:Genes
Subjects:
enzymatic dna synthesis
terminal deoxynucleotidyl transferase
tdt
secondary structures
oligonucleotide synthesis
template-independent polymerase
dna data storage
thermostability engineering
polymerase cofactors
Online Access:https://www.mdpi.com/2073-4425/11/1/102

Internet

https://www.mdpi.com/2073-4425/11/1/102

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