Imaging Membrane Curvature inside a FcεRI-Centric Synapse in RBL-2H3 Cells Using TIRF Microscopy with Polarized Excitation

Imaging Membrane Curvature inside a FcεRI-Centric Synapse in RBL-2H3 Cells Using TIRF Microscopy with Polarized Excitation

Total internal reflection fluorescence microscopy with polarized excitation (P-TIRF) can be used to image nanoscale curvature phenomena in live cells. We used P-TIRF to visualize rat basophilic leukemia cells (RBL-2H3 cells) primed with fluorescent anti-dinitrophenyl (anti-DNP) immunoglobulin E (IgE...

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Bibliographic Details
Main Authors: Rosa Machado, Justin Bendesky, Madison Brown, Kathrin Spendier, Guy M. Hagen
Format: Article
Language:English
Published: MDPI AG 2019-07-01
Series:Journal of Imaging
Subjects:
total internal reflection fluorescence microscopy
P-TIRF
rat basophilic leukemia cells
RBL-2H3
IgE receptor
FcεRI
plasma membrane
supported lipid bilayer
Online Access:https://www.mdpi.com/2313-433X/5/7/63

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https://www.mdpi.com/2313-433X/5/7/63

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