Depleting RhoA/Stress Fiber-Organized Fibronectin Matrices on Tumor Cells Non-Autonomously Aggravates Fibroblast-Driven Tumor Cell Growth
Fibronectin (FN) expressed by tumor cells has been known to be tumor suppressive but the pericellular FN (periFN) assembled on circulating tumor cells appears to evidently promote distant metastasis. Whereas the regulation of periFN assembly in suspended cells has currently been under investigation,...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-11-01
|
Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | https://www.mdpi.com/1422-0067/21/21/8272 |
id |
doaj-05974621d6ea4b1abab605257f4a3511 |
---|---|
record_format |
Article |
spelling |
doaj-05974621d6ea4b1abab605257f4a35112020-11-25T04:06:53ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-11-01218272827210.3390/ijms21218272Depleting RhoA/Stress Fiber-Organized Fibronectin Matrices on Tumor Cells Non-Autonomously Aggravates Fibroblast-Driven Tumor Cell GrowthLi-Tzu Huang0Chen-Lung Tsai1Shin-Huei Huang2Ming-Min Chang3Wen-Tsan Chang4Li-Hsin Cheng5Hung-Chi Cheng6The Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 1 University Road, Tainan 70101, TaiwanDepartment of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, 1 University Road, Tainan 70101, TaiwanDepartment of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, 1 University Road, Tainan 70101, TaiwanThe Institute of Clinical Pharmacy and Pharmaceutical Science, College of Medicine, National Cheng Kung University, 1 University Road, Tainan 70101, TaiwanThe Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 1 University Road, Tainan 70101, TaiwanThe Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 1 University Road, Tainan 70101, TaiwanThe Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 1 University Road, Tainan 70101, TaiwanFibronectin (FN) expressed by tumor cells has been known to be tumor suppressive but the pericellular FN (periFN) assembled on circulating tumor cells appears to evidently promote distant metastasis. Whereas the regulation of periFN assembly in suspended cells has currently been under investigation, how it is regulated in adherent tumor cells and the role of periFN in primary tumor growth remain elusive. Techniques of RNAi, plasmid transfections, immunoblotting, fluorescence/immunohistochemistry staining, cell proliferation assays, and primary tumor growth in C57BL6 mice and Fischer 344 rats were employed in this study. We found that endogenously synthesized FN in adherent tumor cells was required for periFN assembly which was aligned by RhoA-organized actin stress fiber (SF). Depleting periFN on adherent tumor cells congruently promoted in vivo tumor growth but surprisingly did not autonomously impact on in vitro tumor cell proliferation and apoptosis, suggestive of a non-autonomous role of periFN in in vivo tumor growth. We showed that the proliferative ability of shFN-expressing tumor cells was higher than shScramble cells did in the presence of fibroblasts. Altogether, these results suggested that depriving RhoA/SF-regulated periFN matrices non-autonomously promotes fibroblast-mediated tumor cell growth.https://www.mdpi.com/1422-0067/21/21/8272Fibronectin (FN)pericellular FN matricesactin stress fiber cytoskeletonRhoAtumor microenvironmentscancer associated fibroblasts |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Li-Tzu Huang Chen-Lung Tsai Shin-Huei Huang Ming-Min Chang Wen-Tsan Chang Li-Hsin Cheng Hung-Chi Cheng |
spellingShingle |
Li-Tzu Huang Chen-Lung Tsai Shin-Huei Huang Ming-Min Chang Wen-Tsan Chang Li-Hsin Cheng Hung-Chi Cheng Depleting RhoA/Stress Fiber-Organized Fibronectin Matrices on Tumor Cells Non-Autonomously Aggravates Fibroblast-Driven Tumor Cell Growth International Journal of Molecular Sciences Fibronectin (FN) pericellular FN matrices actin stress fiber cytoskeleton RhoA tumor microenvironments cancer associated fibroblasts |
author_facet |
Li-Tzu Huang Chen-Lung Tsai Shin-Huei Huang Ming-Min Chang Wen-Tsan Chang Li-Hsin Cheng Hung-Chi Cheng |
author_sort |
Li-Tzu Huang |
title |
Depleting RhoA/Stress Fiber-Organized Fibronectin Matrices on Tumor Cells Non-Autonomously Aggravates Fibroblast-Driven Tumor Cell Growth |
title_short |
Depleting RhoA/Stress Fiber-Organized Fibronectin Matrices on Tumor Cells Non-Autonomously Aggravates Fibroblast-Driven Tumor Cell Growth |
title_full |
Depleting RhoA/Stress Fiber-Organized Fibronectin Matrices on Tumor Cells Non-Autonomously Aggravates Fibroblast-Driven Tumor Cell Growth |
title_fullStr |
Depleting RhoA/Stress Fiber-Organized Fibronectin Matrices on Tumor Cells Non-Autonomously Aggravates Fibroblast-Driven Tumor Cell Growth |
title_full_unstemmed |
Depleting RhoA/Stress Fiber-Organized Fibronectin Matrices on Tumor Cells Non-Autonomously Aggravates Fibroblast-Driven Tumor Cell Growth |
title_sort |
depleting rhoa/stress fiber-organized fibronectin matrices on tumor cells non-autonomously aggravates fibroblast-driven tumor cell growth |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2020-11-01 |
description |
Fibronectin (FN) expressed by tumor cells has been known to be tumor suppressive but the pericellular FN (periFN) assembled on circulating tumor cells appears to evidently promote distant metastasis. Whereas the regulation of periFN assembly in suspended cells has currently been under investigation, how it is regulated in adherent tumor cells and the role of periFN in primary tumor growth remain elusive. Techniques of RNAi, plasmid transfections, immunoblotting, fluorescence/immunohistochemistry staining, cell proliferation assays, and primary tumor growth in C57BL6 mice and Fischer 344 rats were employed in this study. We found that endogenously synthesized FN in adherent tumor cells was required for periFN assembly which was aligned by RhoA-organized actin stress fiber (SF). Depleting periFN on adherent tumor cells congruently promoted in vivo tumor growth but surprisingly did not autonomously impact on in vitro tumor cell proliferation and apoptosis, suggestive of a non-autonomous role of periFN in in vivo tumor growth. We showed that the proliferative ability of shFN-expressing tumor cells was higher than shScramble cells did in the presence of fibroblasts. Altogether, these results suggested that depriving RhoA/SF-regulated periFN matrices non-autonomously promotes fibroblast-mediated tumor cell growth. |
topic |
Fibronectin (FN) pericellular FN matrices actin stress fiber cytoskeleton RhoA tumor microenvironments cancer associated fibroblasts |
url |
https://www.mdpi.com/1422-0067/21/21/8272 |
work_keys_str_mv |
AT litzuhuang depletingrhoastressfiberorganizedfibronectinmatricesontumorcellsnonautonomouslyaggravatesfibroblastdriventumorcellgrowth AT chenlungtsai depletingrhoastressfiberorganizedfibronectinmatricesontumorcellsnonautonomouslyaggravatesfibroblastdriventumorcellgrowth AT shinhueihuang depletingrhoastressfiberorganizedfibronectinmatricesontumorcellsnonautonomouslyaggravatesfibroblastdriventumorcellgrowth AT mingminchang depletingrhoastressfiberorganizedfibronectinmatricesontumorcellsnonautonomouslyaggravatesfibroblastdriventumorcellgrowth AT wentsanchang depletingrhoastressfiberorganizedfibronectinmatricesontumorcellsnonautonomouslyaggravatesfibroblastdriventumorcellgrowth AT lihsincheng depletingrhoastressfiberorganizedfibronectinmatricesontumorcellsnonautonomouslyaggravatesfibroblastdriventumorcellgrowth AT hungchicheng depletingrhoastressfiberorganizedfibronectinmatricesontumorcellsnonautonomouslyaggravatesfibroblastdriventumorcellgrowth |
_version_ |
1724430342150946816 |