Interphase Chromosomes in Replicative Senescence: Chromosome Positioning as a Senescence Biomarker and the Lack of Nuclear Motor-Driven Chromosome Repositioning in Senescent Cells
This study demonstrates, and confirms, that chromosome territory positioning is altered in primary senescent human dermal fibroblasts (HDFs). The chromosome territory positioning pattern is very similar to that found in HDFs made quiescent either by serum starvation or confluence; but not completely...
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2021-05-01
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doaj-05d60265ec75408abded1657357ca06f2021-05-25T11:08:23ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-05-01910.3389/fcell.2021.640200640200Interphase Chromosomes in Replicative Senescence: Chromosome Positioning as a Senescence Biomarker and the Lack of Nuclear Motor-Driven Chromosome Repositioning in Senescent CellsIshita S. Mehta0Ishita S. Mehta1Kumars Riyahi2Rita Torres Pereira3Karen J. Meaburn4Martin Figgitt5Martin Figgitt6Ian R. Kill7Christopher H. Eskiw8Joanna M. Bridger9Centre for Genome Engineering and Maintenance, Division of Biosciences, Department of Life Sciences, College of Health, Medicine and Life Sciences, Kingston Lane, Brunel UniversityLondon, Uxbridge, United KingdomTata Institute of Fundamental Research, Mumbai, IndiaCentre for Genome Engineering and Maintenance, Division of Biosciences, Department of Life Sciences, College of Health, Medicine and Life Sciences, Kingston Lane, Brunel UniversityLondon, Uxbridge, United KingdomCentre for Genome Engineering and Maintenance, Division of Biosciences, Department of Life Sciences, College of Health, Medicine and Life Sciences, Kingston Lane, Brunel UniversityLondon, Uxbridge, United KingdomCentre for Genome Engineering and Maintenance, Division of Biosciences, Department of Life Sciences, College of Health, Medicine and Life Sciences, Kingston Lane, Brunel UniversityLondon, Uxbridge, United KingdomCentre for Genome Engineering and Maintenance, Division of Biosciences, Department of Life Sciences, College of Health, Medicine and Life Sciences, Kingston Lane, Brunel UniversityLondon, Uxbridge, United KingdomDepartment of Life Sciences, Birmingham City University, Birmingham, United KingdomCentre for Genome Engineering and Maintenance, Division of Biosciences, Department of Life Sciences, College of Health, Medicine and Life Sciences, Kingston Lane, Brunel UniversityLondon, Uxbridge, United KingdomDepartment of Food and Bioproduct Sciences, University of Saskatchewan, Saskatoon, SK, CanadaCentre for Genome Engineering and Maintenance, Division of Biosciences, Department of Life Sciences, College of Health, Medicine and Life Sciences, Kingston Lane, Brunel UniversityLondon, Uxbridge, United KingdomThis study demonstrates, and confirms, that chromosome territory positioning is altered in primary senescent human dermal fibroblasts (HDFs). The chromosome territory positioning pattern is very similar to that found in HDFs made quiescent either by serum starvation or confluence; but not completely. A few chromosomes are found in different locations. One chromosome in particular stands out, chromosome 10, which is located in an intermediate location in young proliferating HDFs, but is found at the nuclear periphery in quiescent cells and in an opposing location of the nuclear interior in senescent HDFs. We have previously demonstrated that individual chromosome territories can be actively and rapidly relocated, with 15 min, after removal of serum from the culture media. These chromosome relocations require nuclear motor activity through the presence of nuclear myosin 1β (NM1β). We now also demonstrate rapid chromosome movement in HDFs after heat-shock at 42°C. Others have shown that heat shock genes are actively relocated using nuclear motor protein activity via actin or NM1β (Khanna et al., 2014; Pradhan et al., 2020). However, this current study reveals, that in senescent HDFs, chromosomes can no longer be relocated to expected nuclear locations upon these two types of stimuli. This coincides with a entirely different organisation and distribution of NM1β within senescent HDFs.https://www.frontiersin.org/articles/10.3389/fcell.2021.640200/fullreplicative senescence (RS)genome organisationnuclear motorschromatin dynamicschromosome territoriesnuclear myosin 1β |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ishita S. Mehta Ishita S. Mehta Kumars Riyahi Rita Torres Pereira Karen J. Meaburn Martin Figgitt Martin Figgitt Ian R. Kill Christopher H. Eskiw Joanna M. Bridger |
spellingShingle |
Ishita S. Mehta Ishita S. Mehta Kumars Riyahi Rita Torres Pereira Karen J. Meaburn Martin Figgitt Martin Figgitt Ian R. Kill Christopher H. Eskiw Joanna M. Bridger Interphase Chromosomes in Replicative Senescence: Chromosome Positioning as a Senescence Biomarker and the Lack of Nuclear Motor-Driven Chromosome Repositioning in Senescent Cells Frontiers in Cell and Developmental Biology replicative senescence (RS) genome organisation nuclear motors chromatin dynamics chromosome territories nuclear myosin 1β |
author_facet |
Ishita S. Mehta Ishita S. Mehta Kumars Riyahi Rita Torres Pereira Karen J. Meaburn Martin Figgitt Martin Figgitt Ian R. Kill Christopher H. Eskiw Joanna M. Bridger |
author_sort |
Ishita S. Mehta |
title |
Interphase Chromosomes in Replicative Senescence: Chromosome Positioning as a Senescence Biomarker and the Lack of Nuclear Motor-Driven Chromosome Repositioning in Senescent Cells |
title_short |
Interphase Chromosomes in Replicative Senescence: Chromosome Positioning as a Senescence Biomarker and the Lack of Nuclear Motor-Driven Chromosome Repositioning in Senescent Cells |
title_full |
Interphase Chromosomes in Replicative Senescence: Chromosome Positioning as a Senescence Biomarker and the Lack of Nuclear Motor-Driven Chromosome Repositioning in Senescent Cells |
title_fullStr |
Interphase Chromosomes in Replicative Senescence: Chromosome Positioning as a Senescence Biomarker and the Lack of Nuclear Motor-Driven Chromosome Repositioning in Senescent Cells |
title_full_unstemmed |
Interphase Chromosomes in Replicative Senescence: Chromosome Positioning as a Senescence Biomarker and the Lack of Nuclear Motor-Driven Chromosome Repositioning in Senescent Cells |
title_sort |
interphase chromosomes in replicative senescence: chromosome positioning as a senescence biomarker and the lack of nuclear motor-driven chromosome repositioning in senescent cells |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cell and Developmental Biology |
issn |
2296-634X |
publishDate |
2021-05-01 |
description |
This study demonstrates, and confirms, that chromosome territory positioning is altered in primary senescent human dermal fibroblasts (HDFs). The chromosome territory positioning pattern is very similar to that found in HDFs made quiescent either by serum starvation or confluence; but not completely. A few chromosomes are found in different locations. One chromosome in particular stands out, chromosome 10, which is located in an intermediate location in young proliferating HDFs, but is found at the nuclear periphery in quiescent cells and in an opposing location of the nuclear interior in senescent HDFs. We have previously demonstrated that individual chromosome territories can be actively and rapidly relocated, with 15 min, after removal of serum from the culture media. These chromosome relocations require nuclear motor activity through the presence of nuclear myosin 1β (NM1β). We now also demonstrate rapid chromosome movement in HDFs after heat-shock at 42°C. Others have shown that heat shock genes are actively relocated using nuclear motor protein activity via actin or NM1β (Khanna et al., 2014; Pradhan et al., 2020). However, this current study reveals, that in senescent HDFs, chromosomes can no longer be relocated to expected nuclear locations upon these two types of stimuli. This coincides with a entirely different organisation and distribution of NM1β within senescent HDFs. |
topic |
replicative senescence (RS) genome organisation nuclear motors chromatin dynamics chromosome territories nuclear myosin 1β |
url |
https://www.frontiersin.org/articles/10.3389/fcell.2021.640200/full |
work_keys_str_mv |
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