Development of Immunoassay Based on Monoclonal Antibody Reacted with the Neonicotinoid Insecticides Clothianidin and Dinotefuran

Enzyme-linked immunosorbent assay (ELISA) based on a monoclonal antibody (MoAb) was developed for the neonicotinoid insecticide clothianidin. A new clothianidin hapten (3-[5-(3-methyl-2-nitroguanidinomethyl)-1,3-thiazol-2-ylthio] propionic acid) was synthesized and conjugated to keyhole limpet hemoc...

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Bibliographic Details
Main Authors: Seiji Iwasa, Mikiko Uchigashima, Shigekazu Ito, Eiki Watanabe, Shiro Miyake
Format: Article
Language:English
Published: MDPI AG 2012-11-01
Series:Sensors
Subjects:
Online Access:http://www.mdpi.com/1424-8220/12/11/15858