A sulfhydryl-reactive ruthenium (II) complex and its conjugation to protein G as a universal reagent for fluorescent immunoassays.

A sulfhydryl-reactive ruthenium (II) complex and its conjugation to protein G as a universal reagent for fluorescent immunoassays.

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To develop a fluorescent ruthenium complex for biosensing, we synthesized a novel sulfhydryl-reactive compound, 4-bromophenanthroline bis-2,2'-dipyridine Ruthenium bis (hexafluorophosphate). The synthesized Ru(II) complex was crosslinked with thiol-modified protein G to form a universal reagent...

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Bibliographic Details
Main Authors: Jing-Tang Lin, Po-Chung Chen, Thirumani Venkatshwar Goud, Bor-Rong Huang, Tzu-Chau Lin, Jean-François Biellmann, Chien-Sheng Chen
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3338566?pdf=render

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http://europepmc.org/articles/PMC3338566?pdf=render

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