A Method for the Determination of Bi-substrate Kinetic Coefficients: the Example of the b-D-glucose- NAD-GDH Enzymatic Reaction

Colorimetric detection of glucose in sample liquids such as human plasma is made by using enzymatic reactions. Either glucose oxidase (GOX) or glucose dehydrogenase (GDH) can be used to convert glucose. In the multi reactional scheme, the first enzymatic reaction is determinant. We focused here on t...

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Bibliographic Details
Main Authors: Jean BERTHIER, Myriam Cubizolles, Patricia LAURENT, Vincent POHER, Patrick POUTEAU, Marie-Line COSNIER
Format: Article
Language:English
Published: IFSA Publishing, S.L. 2015-08-01
Series:Sensors & Transducers
Subjects:
Online Access:http://www.sensorsportal.com/HTML/DIGEST/august_2015/Vol_191/P_2707.pdf