Development of a CRISPR/Cpf1 system for targeted gene disruption in Aspergillus aculeatus TBRC 277

Development of a CRISPR/Cpf1 system for targeted gene disruption in Aspergillus aculeatus TBRC 277

Abstract Background CRISPR-Cas genome editing technologies have revolutionized biotechnological research particularly in functional genomics and synthetic biology. As an alternative to the most studied and well-developed CRISPR/Cas9, a new class 2 (type V) CRISPR-Cas system called Cpf1 has emerged a...

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Bibliographic Details
Main Authors: Dede Abdulrachman, Lily Eurwilaichitr, Verawat Champreda, Duriya Chantasingh, Kusol Pootanakit
Format: Article
Language:English
Published: BMC 2021-02-01
Series:BMC Biotechnology
Subjects:
CRISPR/Cpf1
pyrG
5-FOA
FnCpf1
Gene editing
Filamentous fungi
Online Access:https://doi.org/10.1186/s12896-021-00669-8

Internet

https://doi.org/10.1186/s12896-021-00669-8

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