Efficient CRISPR/Cas9 Genome Editing of Phytoene desaturase in Cassava

CRISPR/Cas9 has become a powerful genome-editing tool for introducing genetic changes into crop species. In order to develop capacity for CRISPR/Cas9 technology in the tropical staple cassava (Manihot esculenta), the Phytoene desaturase (MePDS) gene was targeted in two cultivars using constructs car...

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Bibliographic Details
Main Authors: John Odipio, Titus Alicai, Ivan Ingelbrecht, Dmitri A. Nusinow, Rebecca Bart, Nigel J. Taylor
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-10-01
Series:Frontiers in Plant Science
Subjects:
Online Access:http://journal.frontiersin.org/article/10.3389/fpls.2017.01780/full