Phosphorylation Modulates the Subcellular Localization of SOX11
SOX11 is a key Transcription Factor (TF) in the regulation of embryonic and adult neurogenesis, whose mutation has recently been linked to an intellectual disability syndrome in humans. SOX11’s transient activity during neurogenesis is critical to ensure the precise execution of the neurogenic progr...
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doaj-06b6ae3ed48449bc90521010c660342c2020-11-25T01:43:17ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992018-06-011110.3389/fnmol.2018.00211349650Phosphorylation Modulates the Subcellular Localization of SOX11Elli-Anna Balta0Marie-Theres Wittmann1Matthias Jung2Elisabeth Sock3Benjamin Martin Haeberle4Birgit Heim5Felix von Zweydorf6Jana Heppt7Julia von Wittgenstein8Christian Johannes Gloeckner9Christian Johannes Gloeckner10Dieter Chichung Lie11Institute of Biochemistry, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, GermanyInstitute of Biochemistry, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, GermanyInstitute of Biochemistry, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, GermanyInstitute of Biochemistry, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, GermanyInstitute of Biochemistry, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, GermanyCenter for Ophthalmology, Institute for Ophthalmic Research, University of Tübingen, Tübingen, GermanyDZNE-German Center for Neurodegenerative Diseases, Tübingen, GermanyInstitute of Biochemistry, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, GermanyInstitute of Biochemistry, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, GermanyCenter for Ophthalmology, Institute for Ophthalmic Research, University of Tübingen, Tübingen, GermanyDZNE-German Center for Neurodegenerative Diseases, Tübingen, GermanyInstitute of Biochemistry, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, GermanySOX11 is a key Transcription Factor (TF) in the regulation of embryonic and adult neurogenesis, whose mutation has recently been linked to an intellectual disability syndrome in humans. SOX11’s transient activity during neurogenesis is critical to ensure the precise execution of the neurogenic program. Here, we report that SOX11 displays differential subcellular localizations during the course of neurogenesis. Western-Blot analysis of embryonic mouse brain lysates indicated that SOX11 is post-translationally modified by phosphorylation. Using Mass Spectrometry, we found 10 serine residues in the SOX11 protein that are putatively phosphorylated. Systematic analysis of phospho-mutant SOX11 resulted in the identification of the S30 residue, whose phosphorylation promotes nuclear over cytoplasmic localization of SOX11. Collectively, these findings uncover phosphorylation as a novel layer of regulation of the intellectual disability gene Sox11.https://www.frontiersin.org/article/10.3389/fnmol.2018.00211/fullSOX11transcription factor phosphorylationsubcellular localizationneurogenesiscancerintellectual disability |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Elli-Anna Balta Marie-Theres Wittmann Matthias Jung Elisabeth Sock Benjamin Martin Haeberle Birgit Heim Felix von Zweydorf Jana Heppt Julia von Wittgenstein Christian Johannes Gloeckner Christian Johannes Gloeckner Dieter Chichung Lie |
spellingShingle |
Elli-Anna Balta Marie-Theres Wittmann Matthias Jung Elisabeth Sock Benjamin Martin Haeberle Birgit Heim Felix von Zweydorf Jana Heppt Julia von Wittgenstein Christian Johannes Gloeckner Christian Johannes Gloeckner Dieter Chichung Lie Phosphorylation Modulates the Subcellular Localization of SOX11 Frontiers in Molecular Neuroscience SOX11 transcription factor phosphorylation subcellular localization neurogenesis cancer intellectual disability |
author_facet |
Elli-Anna Balta Marie-Theres Wittmann Matthias Jung Elisabeth Sock Benjamin Martin Haeberle Birgit Heim Felix von Zweydorf Jana Heppt Julia von Wittgenstein Christian Johannes Gloeckner Christian Johannes Gloeckner Dieter Chichung Lie |
author_sort |
Elli-Anna Balta |
title |
Phosphorylation Modulates the Subcellular Localization of SOX11 |
title_short |
Phosphorylation Modulates the Subcellular Localization of SOX11 |
title_full |
Phosphorylation Modulates the Subcellular Localization of SOX11 |
title_fullStr |
Phosphorylation Modulates the Subcellular Localization of SOX11 |
title_full_unstemmed |
Phosphorylation Modulates the Subcellular Localization of SOX11 |
title_sort |
phosphorylation modulates the subcellular localization of sox11 |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Molecular Neuroscience |
issn |
1662-5099 |
publishDate |
2018-06-01 |
description |
SOX11 is a key Transcription Factor (TF) in the regulation of embryonic and adult neurogenesis, whose mutation has recently been linked to an intellectual disability syndrome in humans. SOX11’s transient activity during neurogenesis is critical to ensure the precise execution of the neurogenic program. Here, we report that SOX11 displays differential subcellular localizations during the course of neurogenesis. Western-Blot analysis of embryonic mouse brain lysates indicated that SOX11 is post-translationally modified by phosphorylation. Using Mass Spectrometry, we found 10 serine residues in the SOX11 protein that are putatively phosphorylated. Systematic analysis of phospho-mutant SOX11 resulted in the identification of the S30 residue, whose phosphorylation promotes nuclear over cytoplasmic localization of SOX11. Collectively, these findings uncover phosphorylation as a novel layer of regulation of the intellectual disability gene Sox11. |
topic |
SOX11 transcription factor phosphorylation subcellular localization neurogenesis cancer intellectual disability |
url |
https://www.frontiersin.org/article/10.3389/fnmol.2018.00211/full |
work_keys_str_mv |
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