Targeting HER2-breast tumors with scFv-decorated bimodal nanoprobes
Abstract Background Recent advances in nanomedicine have shown the great interest of active targeting associated to nanoparticles. Single chain variable fragments (scFv) of disease-specific antibodies are very promising targeting entities because they are small, not immunogenic and able to bind thei...
Main Authors: | , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2018-02-01
|
Series: | Journal of Nanobiotechnology |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12951-018-0341-6 |
id |
doaj-06e32453fa744401a32cc90de67b3661 |
---|---|
record_format |
Article |
spelling |
doaj-06e32453fa744401a32cc90de67b36612020-11-24T23:56:10ZengBMCJournal of Nanobiotechnology1477-31552018-02-0116111310.1186/s12951-018-0341-6Targeting HER2-breast tumors with scFv-decorated bimodal nanoprobesChristophe Alric0Katel Hervé-Aubert1Nicolas Aubrey2Souad Melouk3Laurie Lajoie4William Même5Sandra Même6Yann Courbebaisse7Anastasia A. Ignatova8Alexey V. Feofanov9Igor Chourpa10Emilie Allard-Vannier11EA6295 ‘Nanomédicaments et Nanosondes’, Université de ToursEA6295 ‘Nanomédicaments et Nanosondes’, Université de ToursISP, Université de Tours, INRA, UMR 1282, Equipe BIOMédicaments Anti-ParasitairesEA6295 ‘Nanomédicaments et Nanosondes’, Université de ToursGICC ‘Groupe Innovation et Ciblage Cellulaire’, Université de Tours, Equipe FRAME - Fc Récepteurs, Anticorps et MicroEnvironnementCBM, CNRS, UPR4301, Equipe Complexes Métalliques et IRM pour applications biomédicalesCBM, CNRS, UPR4301, Equipe Complexes Métalliques et IRM pour applications biomédicalesBertin Pharma OrléansShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of SciencesShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of SciencesEA6295 ‘Nanomédicaments et Nanosondes’, Université de ToursEA6295 ‘Nanomédicaments et Nanosondes’, Université de ToursAbstract Background Recent advances in nanomedicine have shown the great interest of active targeting associated to nanoparticles. Single chain variable fragments (scFv) of disease-specific antibodies are very promising targeting entities because they are small, not immunogenic and able to bind their specific antigens. The present paper is devoted to biological properties in vitro and in vivo of fluorescent and pegylated iron oxide nanoparticles (SPIONs-Cy-PEG-scFv) functionalized with scFv targeting Human Epithelial growth Receptor 2 (HER2). Results Thanks to a site-selective scFv conjugation, the resultant nanoprobes demonstrated high affinity and specific binding to HER2 breast cancer cells. The cellular uptake of SPIONs-Cy-PEG-scFv was threefold higher than that for untargeted PEGylated iron oxide nanoparticles (SPIONs-Cy-PEG) and is correlated to the expression of HER2 on cells. In vivo, the decrease of MR signals in HER2+ xenograft tumor is about 30% at 24 h after the injection. Conclusions These results all indicate that SPIONs-Cy-PEG-scFv are relevant tumor-targeting magnetic resonance imaging agents, suitable for diagnosis of HER2 overexpressing breast tumor.http://link.springer.com/article/10.1186/s12951-018-0341-6Breast tumorHuman Epithelial growth Receptor 2 (HER2)Iron oxide nanoparticleCyanine 5.5Single chain variable fragment (scFv)Magnetic Resonance Imaging (MRI) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Christophe Alric Katel Hervé-Aubert Nicolas Aubrey Souad Melouk Laurie Lajoie William Même Sandra Même Yann Courbebaisse Anastasia A. Ignatova Alexey V. Feofanov Igor Chourpa Emilie Allard-Vannier |
spellingShingle |
Christophe Alric Katel Hervé-Aubert Nicolas Aubrey Souad Melouk Laurie Lajoie William Même Sandra Même Yann Courbebaisse Anastasia A. Ignatova Alexey V. Feofanov Igor Chourpa Emilie Allard-Vannier Targeting HER2-breast tumors with scFv-decorated bimodal nanoprobes Journal of Nanobiotechnology Breast tumor Human Epithelial growth Receptor 2 (HER2) Iron oxide nanoparticle Cyanine 5.5 Single chain variable fragment (scFv) Magnetic Resonance Imaging (MRI) |
author_facet |
Christophe Alric Katel Hervé-Aubert Nicolas Aubrey Souad Melouk Laurie Lajoie William Même Sandra Même Yann Courbebaisse Anastasia A. Ignatova Alexey V. Feofanov Igor Chourpa Emilie Allard-Vannier |
author_sort |
Christophe Alric |
title |
Targeting HER2-breast tumors with scFv-decorated bimodal nanoprobes |
title_short |
Targeting HER2-breast tumors with scFv-decorated bimodal nanoprobes |
title_full |
Targeting HER2-breast tumors with scFv-decorated bimodal nanoprobes |
title_fullStr |
Targeting HER2-breast tumors with scFv-decorated bimodal nanoprobes |
title_full_unstemmed |
Targeting HER2-breast tumors with scFv-decorated bimodal nanoprobes |
title_sort |
targeting her2-breast tumors with scfv-decorated bimodal nanoprobes |
publisher |
BMC |
series |
Journal of Nanobiotechnology |
issn |
1477-3155 |
publishDate |
2018-02-01 |
description |
Abstract Background Recent advances in nanomedicine have shown the great interest of active targeting associated to nanoparticles. Single chain variable fragments (scFv) of disease-specific antibodies are very promising targeting entities because they are small, not immunogenic and able to bind their specific antigens. The present paper is devoted to biological properties in vitro and in vivo of fluorescent and pegylated iron oxide nanoparticles (SPIONs-Cy-PEG-scFv) functionalized with scFv targeting Human Epithelial growth Receptor 2 (HER2). Results Thanks to a site-selective scFv conjugation, the resultant nanoprobes demonstrated high affinity and specific binding to HER2 breast cancer cells. The cellular uptake of SPIONs-Cy-PEG-scFv was threefold higher than that for untargeted PEGylated iron oxide nanoparticles (SPIONs-Cy-PEG) and is correlated to the expression of HER2 on cells. In vivo, the decrease of MR signals in HER2+ xenograft tumor is about 30% at 24 h after the injection. Conclusions These results all indicate that SPIONs-Cy-PEG-scFv are relevant tumor-targeting magnetic resonance imaging agents, suitable for diagnosis of HER2 overexpressing breast tumor. |
topic |
Breast tumor Human Epithelial growth Receptor 2 (HER2) Iron oxide nanoparticle Cyanine 5.5 Single chain variable fragment (scFv) Magnetic Resonance Imaging (MRI) |
url |
http://link.springer.com/article/10.1186/s12951-018-0341-6 |
work_keys_str_mv |
AT christophealric targetingher2breasttumorswithscfvdecoratedbimodalnanoprobes AT katelherveaubert targetingher2breasttumorswithscfvdecoratedbimodalnanoprobes AT nicolasaubrey targetingher2breasttumorswithscfvdecoratedbimodalnanoprobes AT souadmelouk targetingher2breasttumorswithscfvdecoratedbimodalnanoprobes AT laurielajoie targetingher2breasttumorswithscfvdecoratedbimodalnanoprobes AT williammeme targetingher2breasttumorswithscfvdecoratedbimodalnanoprobes AT sandrameme targetingher2breasttumorswithscfvdecoratedbimodalnanoprobes AT yanncourbebaisse targetingher2breasttumorswithscfvdecoratedbimodalnanoprobes AT anastasiaaignatova targetingher2breasttumorswithscfvdecoratedbimodalnanoprobes AT alexeyvfeofanov targetingher2breasttumorswithscfvdecoratedbimodalnanoprobes AT igorchourpa targetingher2breasttumorswithscfvdecoratedbimodalnanoprobes AT emilieallardvannier targetingher2breasttumorswithscfvdecoratedbimodalnanoprobes |
_version_ |
1725459239950876672 |